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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2-4
|
| pubmed:dateCreated |
1998-7-24
|
| pubmed:abstractText |
An avian pathogenic Escherichia coli strain M1000 showed a clear zone of erythrocyte lysis on sheep blood agar plates. The hemolytic activity was not detected in the culture supernatant nor was any DNA sequence homologous to the E. coli alpha-hemolysin gene detected in the chromosome or plasmid DNA of the strain, indicating that the observed hemolysis was different from alpha-type. To identify the genetic determinant responsible for the hemolysis, we performed random Tn5 insertional mutagenesis and obtained one mutant, named M5005, that totally lacked the hemolytic activity. Cloning and nucleotide sequencing of the region flanking the transposon insertion site in the M5005 chromosome revealed that the transposon was inserted within an open reading frame of the cyclic AMP receptor protein (CRP) gene, which is involved in one of the global regulatory networks of gene expression in E. coli. Nucleotide sequence analysis of the intact crp gene of the strain M1000 showed that the CRP protein of M1000 is 99% identical to that of K-12. Introduction of the intact crp gene on a low copy plasmid into the mutant M5005 restored the hemolytic phenotype, confirming that the mutation site in M5005 was in the crp gene. CRP plays a central role in catabolite repression, the phenomenon by which the synthesis of many enzymes required to metabolize various sugars is repressed in the presence of glucose. When the hemolytic activity of E. coli M1000 grown in the presence of glucose was examined, the hemolysis was totally impaired. These results indicate that the avian pathogenic E. coli strain M1000 produces a hemolysin the expression of which is dependent on crp gene function.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0378-1135
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
28
|
| pubmed:volume |
60
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
227-38
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9646453-Amino Acid Sequence,
pubmed-meshheading:9646453-Animals,
pubmed-meshheading:9646453-Base Sequence,
pubmed-meshheading:9646453-Carrier Proteins,
pubmed-meshheading:9646453-Chickens,
pubmed-meshheading:9646453-Cloning, Molecular,
pubmed-meshheading:9646453-Cyclic AMP Receptor Protein,
pubmed-meshheading:9646453-Escherichia coli,
pubmed-meshheading:9646453-Escherichia coli Infections,
pubmed-meshheading:9646453-Gene Expression Regulation, Bacterial,
pubmed-meshheading:9646453-Hemolysin Proteins,
pubmed-meshheading:9646453-Hemolysis,
pubmed-meshheading:9646453-Molecular Sequence Data,
pubmed-meshheading:9646453-Mutagenesis, Insertional,
pubmed-meshheading:9646453-Polymerase Chain Reaction,
pubmed-meshheading:9646453-Poultry Diseases,
pubmed-meshheading:9646453-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:9646453-Sequence Alignment,
pubmed-meshheading:9646453-Sequence Homology, Amino Acid,
pubmed-meshheading:9646453-Sequence Homology, Nucleic Acid
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
An avian pathogenic Escherichia coli strain produces a hemolysin, the expression of which is dependent on cyclic AMP receptor protein gene function.
|
| pubmed:affiliation |
Nippon Institute for Biological Science, Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article
|