Linked Life Data

9646229

Source:http://linkedlifedata.com/resource/pubmed/id/9646229

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-10-15
pubmed:abstractText
A serie of 29 human normal, benign and malignant breast tissues were cultivated in an attempt to isolate and propagate primary breast epithelial cells in vitro. Explants methodology as well as disaggregation techniques (enzymatic and mechanical) were employed to obtain better culture conditions. Cells derived from breast malignant tissue were propagated in an appropriate and survived in culture for at least 6 months, exhibiting a marked preference to grow in suspension (independent anchorage). Tumorigenicity assays in nude mice were performed with malignant cells obtained from primary culture cells as well as with cells achieved from successive passages. The rate of long time cell survival from malignant and non-malignant tissues demonstrated the accuracy of the methodology but it also emphasised the need for improving technology to obtain cell lines with long survival.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0392-9078
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19-26
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Assessment of methods for primary tissue culture of human breast epithelia.
pubmed:affiliation
Centro de Investigaciones Immunológicas Básicas y Aplicadas (CINIBA), Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Argentina.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't