Linked Life Data

9643567

Source:http://linkedlifedata.com/resource/pubmed/id/9643567

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5-6
pubmed:dateCreated
1998-9-29
pubmed:abstractText
Marrow stromal cells were evaluated several months after autologous BMT for their capacity to support both normal hemopoiesis and secrete the main growth factors involved in its control, G-CSF, GM-CSF, IL-3 and SCF. Stromal layers (SL) were obtained by long-term marrow cultures (LTMC) established from 15 patients (9 with hematologic malignancies and 6 with solid tumors) 3 months after autologous BMT and were compared to pre-graft patients. After irradiation, both post-graft and pre-graft SL were recharged with the same inoculum of normal marrow cells. As compared to pre-graft values, CFU-GM production on post-graft SL was significantly increased during the first 2 weeks of culture whereas it was decreased from week 3 to week 8. These findings were only observed in patients with hematologic malignancies and not in patients with solid tumors. Growth factor secretion was evaluated by ELISA in the supernatants of unstimulated and IL-1-stimulated SL from 10 post-graft patients, 13 pre-graft patients and 5 normal controls. In any group of patients, IL-3 was undetectable either spontaneously or after IL-1-stimulation. As compared to controls, secretion by IL-1-stimulated SL was not different for GM-CSF in pre- and post-graft patients but tended to be decreased for G-CSF in post-graft patients. SCF secretion, which was not induced by IL-1, appeared dramatically decreased in both pre- and post-graft patients. The capacity of post-graft SL to support CFU-GM growth in LTMC was correlated at week 1 with G-CSF secretion and from week 3 to week 8 with SCF secretion. These results suggest that microenvironment remains qualitatively damaged several months after BMT involving a decreased capacity both to support early hemopoiesis and to secrete SCF, particularly in patients grafted for hemopoietic malignancies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1042-8194
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
533-46
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9643567-Adult, pubmed-meshheading:9643567-Bone Marrow Cells, pubmed-meshheading:9643567-Bone Marrow Transplantation, pubmed-meshheading:9643567-Cell Culture Techniques, pubmed-meshheading:9643567-Cells, Cultured, pubmed-meshheading:9643567-Child, pubmed-meshheading:9643567-Colony-Forming Units Assay, pubmed-meshheading:9643567-Follow-Up Studies, pubmed-meshheading:9643567-Granulocyte Colony-Stimulating Factor, pubmed-meshheading:9643567-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:9643567-Hematologic Neoplasms, pubmed-meshheading:9643567-Hematopoietic Cell Growth Factors, pubmed-meshheading:9643567-Humans, pubmed-meshheading:9643567-Interleukin-1, pubmed-meshheading:9643567-Interleukin-3, pubmed-meshheading:9643567-Middle Aged, pubmed-meshheading:9643567-Neoplasms, pubmed-meshheading:9643567-Stem Cell Factor, pubmed-meshheading:9643567-Stromal Cells, pubmed-meshheading:9643567-Time Factors, pubmed-meshheading:9643567-Transplantation Conditioning
pubmed:year
1998
pubmed:articleTitle
Changes in the functional capacity of marrow stromal cells after autologous bone marrow transplantation.
pubmed:affiliation
Laboratory of Hematology, Bretonneau University Hospital, Tours, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't