Linked Life Data

9643546

Source:http://linkedlifedata.com/resource/pubmed/id/9643546

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-9-10
pubmed:databankReference
pubmed:abstractText
The Bacillus subtilis clpP gene, encoding the proteolytic component of the Clp or Ti protease, was cloned and sequenced. The amount of clpP-specific mRNA increased after heat shock, salt and ethanol stress, as well as after treatment with puromycin. Two transcriptional start sites upstream of the clpP structural gene were identified, preceded by sequences resembling the consensus sequences of promoters recognized by sigmaA and sigmaB transcriptional factors of the B. subtilis RNA polymerase respectively. Transcription initiation occurred predominantly at the putative sigmaA-dependent promoter in exponentially growing cells and was induced under stress conditions. After exposure to stress, initiation of transcription also increased at the sigmaB-dependent promoter, but to a lesser extent, indicating that clpP belongs to a double promoter-controlled subgroup of class III general stress genes in B. subtilis. In a sigB mutant strain, clpP remained heat and stress inducible at the sigmaA-dependent promoter. BgaB-reporter gene fusions, carrying either the sigmaA- or the sigmaB-dependent promoter, showed a higher bgaB induction at the sigmaA-dependent promoter, whereas a significantly lower level of induction was measured at the sigmaB-dependent promoter. The sigmaA-dependent promoter appeared to be crucial for the heat-inducible transcription of clpP. A CIRCE (controlling inverted repeat of chaperone expression) element, the characteristic regulation target of class I heat shock genes such as dnaK and groESL, was not found between the transcriptional and translational start sites. Mutants lacking either the proteolytic component ClpP or the regulatory ATPase component ClpX were phenotypically distinct from the wild type. Both mutants produced chains of elongated cells and exhibited severely impaired growth under stress conditions and starvation. Comparison of two-dimensional protein gels from wild-type cells with those from clpP and clpX mutant cells revealed several changes in the protein pattern. Several proteins, such as GroEL, PpiB, PykA, SucD, YhfP, YqkF, YugJ and YvyD, which were found preferentially in higher amounts in both clpP and clpX mutants, might be potential substrates for the ClpXP protease.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
787-802
pubmed:dateRevised
2009-9-3
pubmed:meshHeading
pubmed-meshheading:9643546-Adenosine Triphosphatases, pubmed-meshheading:9643546-Amino Acid Sequence, pubmed-meshheading:9643546-Artificial Gene Fusion, pubmed-meshheading:9643546-Bacillus subtilis, pubmed-meshheading:9643546-Base Sequence, pubmed-meshheading:9643546-Binding Sites, pubmed-meshheading:9643546-Chromosome Mapping, pubmed-meshheading:9643546-DNA, Bacterial, pubmed-meshheading:9643546-Electrophoresis, Gel, Two-Dimensional, pubmed-meshheading:9643546-Endopeptidase Clp, pubmed-meshheading:9643546-Escherichia coli Proteins, pubmed-meshheading:9643546-Gene Expression Regulation, Bacterial, pubmed-meshheading:9643546-Genes, Bacterial, pubmed-meshheading:9643546-Genes, Reporter, pubmed-meshheading:9643546-Heat-Shock Response, pubmed-meshheading:9643546-Humans, pubmed-meshheading:9643546-Molecular Chaperones, pubmed-meshheading:9643546-Molecular Sequence Data, pubmed-meshheading:9643546-Mutagenesis, pubmed-meshheading:9643546-Peptide Chain Initiation, Translational, pubmed-meshheading:9643546-Promoter Regions, Genetic, pubmed-meshheading:9643546-Sequence Homology, Amino Acid, pubmed-meshheading:9643546-Serine Endopeptidases, pubmed-meshheading:9643546-Stimulation, Chemical, pubmed-meshheading:9643546-Transcription, Genetic, pubmed-meshheading:9643546-beta-Galactosidase
pubmed:year
1998
pubmed:articleTitle
Stress induction of the Bacillus subtilis clpP gene encoding a homologue of the proteolytic component of the Clp protease and the involvement of ClpP and ClpX in stress tolerance.
pubmed:affiliation
Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität, Greifswald, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't