Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1998-8-28
pubmed:abstractText
Synthesis and accumulation of the recently identified prostaglandin F2alpha receptor regulatory protein (FPRP) was found to correlate closely with lipid droplet accumulation by 3T3-L1 preadipose cells. FPRP, a transmembrane glycoprotein, has been shown to regulate the binding of ligand to certain seven-transmembrane receptors. Anti-FPRP immunohistochemistry, Western blotting, and metabolic labeling/immunoprecipitation experiments demonstrated that FPRP was not detectable in undifferentiated 3T3-L1 cells. Interestingly, low levels of FPRP mRNA were detected in the undifferentiated 3T3-L1 cells. After induction of adipose differentiation, FPRP mRNA increased approximately 3 fold whereas FPRP synthesis increased approximately 50 fold. Differentiation induction with either dexamethasone/insulin/isobutylmethylxanthine or the thiazolidinedione derivative ADD 4743 were both effective at inducing FPRP accumulation and accumulation of lipid droplets. By co-immunohistochemical and lipid staining, greater than 99% of the cells accumulating lipid droplets possessed FPRP. FPRP mRNA and protein are also found in rat adipose tissue. Treatment of 3T3-L1 cells with an FPRP anti-sense oligonucleotide during differentiation decreased FPRP accumulation and resulted in a decrease in lipid droplets without altering the level of induction of a late marker of adipocyte differentiation, glycerol-3-phosphate dehydrogenase activity. Transient expression of an FPRP cDNA in undifferentiated 3T3-L1 cells was insufficient to induce lipid droplet accumulation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-2275
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1152-61
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9643346-3T3 Cells, pubmed-meshheading:9643346-Adipocytes, pubmed-meshheading:9643346-Animals, pubmed-meshheading:9643346-Base Sequence, pubmed-meshheading:9643346-Cell Differentiation, pubmed-meshheading:9643346-DNA Primers, pubmed-meshheading:9643346-Mice, pubmed-meshheading:9643346-Neoplasm Proteins, pubmed-meshheading:9643346-Oligonucleotides, Antisense, pubmed-meshheading:9643346-Polymerase Chain Reaction, pubmed-meshheading:9643346-Protein Biosynthesis, pubmed-meshheading:9643346-Proteins, pubmed-meshheading:9643346-RNA, Messenger, pubmed-meshheading:9643346-Rats, pubmed-meshheading:9643346-Receptors, Prostaglandin, pubmed-meshheading:9643346-Recombinant Proteins, pubmed-meshheading:9643346-Time Factors, pubmed-meshheading:9643346-Transcription, Genetic, pubmed-meshheading:9643346-Transfection
pubmed:year
1998
pubmed:articleTitle
Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3-L1 cells.
pubmed:affiliation
Department of Pathology, University of Colorado Health Sciences Center, Denver 80262, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't