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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1998-8-28
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pubmed:abstractText |
Synthesis and accumulation of the recently identified prostaglandin F2alpha receptor regulatory protein (FPRP) was found to correlate closely with lipid droplet accumulation by 3T3-L1 preadipose cells. FPRP, a transmembrane glycoprotein, has been shown to regulate the binding of ligand to certain seven-transmembrane receptors. Anti-FPRP immunohistochemistry, Western blotting, and metabolic labeling/immunoprecipitation experiments demonstrated that FPRP was not detectable in undifferentiated 3T3-L1 cells. Interestingly, low levels of FPRP mRNA were detected in the undifferentiated 3T3-L1 cells. After induction of adipose differentiation, FPRP mRNA increased approximately 3 fold whereas FPRP synthesis increased approximately 50 fold. Differentiation induction with either dexamethasone/insulin/isobutylmethylxanthine or the thiazolidinedione derivative ADD 4743 were both effective at inducing FPRP accumulation and accumulation of lipid droplets. By co-immunohistochemical and lipid staining, greater than 99% of the cells accumulating lipid droplets possessed FPRP. FPRP mRNA and protein are also found in rat adipose tissue. Treatment of 3T3-L1 cells with an FPRP anti-sense oligonucleotide during differentiation decreased FPRP accumulation and resulted in a decrease in lipid droplets without altering the level of induction of a late marker of adipocyte differentiation, glycerol-3-phosphate dehydrogenase activity. Transient expression of an FPRP cDNA in undifferentiated 3T3-L1 cells was insufficient to induce lipid droplet accumulation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ptgfrn protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Prostaglandin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-2275
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1152-61
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9643346-3T3 Cells,
pubmed-meshheading:9643346-Adipocytes,
pubmed-meshheading:9643346-Animals,
pubmed-meshheading:9643346-Base Sequence,
pubmed-meshheading:9643346-Cell Differentiation,
pubmed-meshheading:9643346-DNA Primers,
pubmed-meshheading:9643346-Mice,
pubmed-meshheading:9643346-Neoplasm Proteins,
pubmed-meshheading:9643346-Oligonucleotides, Antisense,
pubmed-meshheading:9643346-Polymerase Chain Reaction,
pubmed-meshheading:9643346-Protein Biosynthesis,
pubmed-meshheading:9643346-Proteins,
pubmed-meshheading:9643346-RNA, Messenger,
pubmed-meshheading:9643346-Rats,
pubmed-meshheading:9643346-Receptors, Prostaglandin,
pubmed-meshheading:9643346-Recombinant Proteins,
pubmed-meshheading:9643346-Time Factors,
pubmed-meshheading:9643346-Transcription, Genetic,
pubmed-meshheading:9643346-Transfection
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pubmed:year |
1998
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pubmed:articleTitle |
Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3-L1 cells.
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pubmed:affiliation |
Department of Pathology, University of Colorado Health Sciences Center, Denver 80262, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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