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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
27
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pubmed:dateCreated |
1998-8-6
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pubmed:abstractText |
Chemoelectrical signal transduction in olfactory neurons appears to involve intracellular reaction cascades mediated by heterotrimeric GTP-binding proteins. In this study attempts were made to identify the G protein subtype(s) in olfactory cilia that are activated by the primary (odorant) signal. Antibodies directed against the alpha subunits of distinct G protein subtypes interfered specifically with second messenger reponses elicited by defined subsets of odorants; odor-induced cAMP-formation was attenuated by Galphas antibodies, whereas Galphao antibodies blocked odor-induced inositol 1,4, 5-trisphosphate (IP3) formation. Activation-dependent photolabeling of Galpha subunits with [alpha-32P]GTP azidoanilide followed by immunoprecipitation using subtype-specific antibodies enabled identification of particular individual G protein subtypes that were activated upon stimulation of isolated olfactory cilia by chemically distinct odorants. For example odorants that elicited a cAMP response resulted in labeling of a Galphas-like protein, whereas odorants that elicited an IP3 response led to the labeling of a Galphao-like protein. Since odorant-induced IP3 formation was also blocked by Gbeta antibodies, activation of olfactory phospholipase C might be mediated by betagamma subunits of a Go-like G protein. These results indicate that different subsets of odorants selectively trigger distinct reaction cascades and provide evidence for dual transduction pathways in olfactory signaling.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Aldehydes,
http://linkedlifedata.com/resource/pubmed/chemical/Benzaldehydes,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclohexenes,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclopentanes,
http://linkedlifedata.com/resource/pubmed/chemical/Eugenol,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Lyral,
http://linkedlifedata.com/resource/pubmed/chemical/Nitriles,
http://linkedlifedata.com/resource/pubmed/chemical/Oxylipins,
http://linkedlifedata.com/resource/pubmed/chemical/Photoaffinity Labels,
http://linkedlifedata.com/resource/pubmed/chemical/citralva,
http://linkedlifedata.com/resource/pubmed/chemical/ethyl vanillin,
http://linkedlifedata.com/resource/pubmed/chemical/lilial,
http://linkedlifedata.com/resource/pubmed/chemical/methyl jasmonate
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
16669-77
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9642220-Acetic Acids,
pubmed-meshheading:9642220-Aldehydes,
pubmed-meshheading:9642220-Amino Acid Sequence,
pubmed-meshheading:9642220-Animals,
pubmed-meshheading:9642220-Benzaldehydes,
pubmed-meshheading:9642220-Cilia,
pubmed-meshheading:9642220-Cyclic AMP,
pubmed-meshheading:9642220-Cyclohexenes,
pubmed-meshheading:9642220-Cyclopentanes,
pubmed-meshheading:9642220-Eugenol,
pubmed-meshheading:9642220-GTP-Binding Proteins,
pubmed-meshheading:9642220-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:9642220-Molecular Sequence Data,
pubmed-meshheading:9642220-Nitriles,
pubmed-meshheading:9642220-Odors,
pubmed-meshheading:9642220-Olfactory Mucosa,
pubmed-meshheading:9642220-Oxylipins,
pubmed-meshheading:9642220-Photoaffinity Labels,
pubmed-meshheading:9642220-Rats,
pubmed-meshheading:9642220-Rats, Sprague-Dawley,
pubmed-meshheading:9642220-Signal Transduction
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pubmed:year |
1998
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pubmed:articleTitle |
Odorants selectively activate distinct G protein subtypes in olfactory cilia.
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pubmed:affiliation |
Universität Stuttgart-Hohenheim, Institut für Physiologie, 70593 Stuttgart, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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