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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1998-7-7
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pubmed:abstractText |
Signalling through the CD2 molecule was shown previously to employ similar signalling molecules as the T-cell receptor (TCR). Here, we show that CD2-mediated signalling is strongly influenced by the expressed transmembrane region of the employed signal-transducing molecule. We used TCR-negative cells expressing chimeric fusion proteins that consist of human interleukin-2 (IL-2) receptor alpha-chain-derived sequences (hCD25) fused to mouse-specific zeta-chain segments (hCD25-zeta). One set of TCR-negative cell lines expressed the hCD25-derived extracellular part fused to mouse-specific transmembrane and cytoplasmic zeta-protein sequences ('TZZ'). The second type of cell lines expressed the hCD25-derived extracellular and transmembrane portions fused to the mouse-specific zeta-chain cytoplasmic segment ('TTZ'). After cross-linking the hCD25-zeta molecules with specific monoclonal antibodies (mAb), all TCR-negative cell lines produced similar amounts of IL-2. Cross-linking with stimulating pairs of CD2-specific mAb, however, led to IL-2 production only in cell lines expressing the zeta-chain-specific transmembrane segment. Co-cross-linking of CD25 and CD2 molecules resulted in an effective stimulation of both TZZ- and TTZ-expressing cell lines. Moreover, TTZ- and TZZ-expressing cell lines differed in their pattern of tyrosine-phosphorylated proteins after stimulation with hCD25-specific mAb. Thus, although CD2 and TCR molecules share signalling components and pathways, the fine tuning of CD2 co-receptor function appears to be regulated in part by transmembrane regions of signal-transducing molecules like the TCR-associated zeta-chain.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-1346934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-1351920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-1377404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-1679714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-1688582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-1705867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-1717631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-1833767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-2142801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-2428504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-2459290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-2558022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-2567497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-2831066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-2901344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-2927501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-3086481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-7681075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-7693851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-7792603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-7843246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-7909722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-8335930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-8551220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-8566064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-8612576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-8977276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-9008160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9640248-9135555
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
376-82
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9640248-Antibodies, Monoclonal,
pubmed-meshheading:9640248-Antigens, CD2,
pubmed-meshheading:9640248-Cells, Cultured,
pubmed-meshheading:9640248-Humans,
pubmed-meshheading:9640248-Hybridomas,
pubmed-meshheading:9640248-Interleukin-2,
pubmed-meshheading:9640248-Lymphocyte Activation,
pubmed-meshheading:9640248-Receptors, Antigen, T-Cell,
pubmed-meshheading:9640248-Receptors, Interleukin-2,
pubmed-meshheading:9640248-Signal Transduction,
pubmed-meshheading:9640248-T-Lymphocytes
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pubmed:year |
1998
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pubmed:articleTitle |
The transmembrane region of CD2-associated signal-transducing proteins is crucial for the outcome of CD2-mediated T-cell activation.
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pubmed:affiliation |
Bernhard-Nocht Institute for Tropical Medicine, Hamburg, Germany.
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pubmed:publicationType |
Journal Article
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