Linked Life Data

9639021

Source:http://linkedlifedata.com/resource/pubmed/id/9639021

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4-5
pubmed:dateCreated
1998-8-19
pubmed:abstractText
Modulation of L-type Ca2+ channels by acetylcholine (ACh) was studied in enzymatically isolated guinea-pig tracheal smooth muscle cells (TSMCs). ACh reversibly inhibited whole cell L type Ca2+ current measured with Ba2+ ions as charge carriers (I(Ba)). With pipette solution containing 0.1 mM EGTA, 1 microM ACh induced transient inhibition of I(Ba) followed by sustained inhibition (67.0+/-3.7% of the control, n=19). When intracellular Ca2+ concentration ([Ca2+]i) was fixed at 50 nM by BAPTA-Ca2+ buffer in the pipette, the transient inhibition was abolished whereas the sustained inhibition (66.0+/-7.8%, n=6) still occurred, suggesting that the transient inhibition was attributed to inactivation of the channels induced by increase in [Ca2+]i. The sustained inhibition was abolished when [Ca2+]i was fixed at zero. The sustained inhibition of I(Ba) by 1 microM ACh was observed in the presence of 10 microM AF-DX 116, whereas it was not observed in the presence of 1 microM 4 DAMP. ACh did not inhibit I(Ba) in the presence of 1 mM GDP-beta-S in the pipette, whereas the drug irreversibly inhibited the current in the presence of 0.1 mM GTP-gamma-S in the pipette. Pretreatment of TSMCs with pertussis toxin did not altered the effects of ACh. Application of neither 1-oleoyl-2-acetyl sn-glycerol (1 microM) nor phorbol 12-myristate 13-acetate (1 microM) reduced I(Ba). These results suggest that the sustained inhibition of I(Ba) by ACh is mediated by Ca2+ requiring and protein kinase C-independent mechanisms existing in the downstream of G-protein coupled with M3 receptors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0916-8737
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
175-85
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:articleTitle
Muscarinic inhibition of L-type Ca2+ channels in guinea-pig tracheal smooth muscle cells.
pubmed:affiliation
Department of Physiology, Nihon University School of Medicine, Tokyo, Japan.
pubmed:publicationType
Journal Article