Linked Life Data

9637018

Source:http://linkedlifedata.com/resource/pubmed/id/9637018

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1998-7-13
pubmed:abstractText
A method is described for the determination of bradykinin, neuropeptide K (NPK), and substance P in patients with atypical carcinoid syndrome. The developed method uses a combination of conventional and solid-phase extraction as well as high-performance liquid chromatographic techniques. A narrow-bore C18 column with ultraviolet detection is used (200 nm). The technique recovers bradykinin at a level of 98%, NPK at 96%, and substance P at 98% (when pure standards are dissolved) at concentration levels relevant to the atypical carcinoid syndrome. In biological samples, the recovery rate of bradykinin, NPK, and substance P drops to 88, 86, and 88% respectively. The overall analysis time is 150 min from receipt of samples. This method proves to be a valuable tool in the identification of neuropeptides and thus the diagnosis of atypical carcinoid syndrome, especially in puzzling cases with nonspecific symptoms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9665
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
306-10
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
High-performance liquid chromatographic analysis with diode-array detection of bradykinin, neuropeptide K, and substance P in human plasma.
pubmed:affiliation
Department of Chemistry and Physics, Technikon Pretoria, South Africa.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't