Linked Life Data

963632

Source:http://linkedlifedata.com/resource/pubmed/id/963632

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1976-11-21
pubmed:abstractText
Heating in potassium phosphate buffer causes Staphylococcus aureus to lose its salt tolerance and 30-40% of its cellular Mg2+. Repair from injury (regain of salt tolerance) occurred when injured cells were incubated under optimal conditions in synthetic media containing penicillin to prevent growth. Cells died when phosphates or amin acids were omitted from the medium. Omission of vitamins, glucose, Na+, and K+ had no effect. Omission of Mg2+ diminished repair. In a minimal repair medium (MRM) which contained only 3 X 10(-6) M Mg (as an impurity), injured cells rapidly regained their original Mg content. About 20-50% of the cells also regained their salt tolerance provided that less than 10(9) cells/ml were used. With 10(10) cells/ml there was no repair and cellular Mg content was half that of the control. Addition of 10(-3) M ethylenediamine-tetraacetic acid (EDTA) to MRM also prevented repair. Addition of 10(-2) M Mg to MRM EDTA permitted complete repair.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0008-4166
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1202-5
pubmed:dateRevised
2009-10-27
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Magnesium requirement of Staphylococcus aureus for repair from sublethal heat injury.
pubmed:publicationType
Journal Article