| pubmed-article:9635844 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9635844 | lifeskim:mentions | umls-concept:C0087111 | lld:lifeskim |
| pubmed-article:9635844 | lifeskim:mentions | umls-concept:C1458155 | lld:lifeskim |
| pubmed-article:9635844 | lifeskim:mentions | umls-concept:C0034804 | lld:lifeskim |
| pubmed-article:9635844 | lifeskim:mentions | umls-concept:C0039286 | lld:lifeskim |
| pubmed-article:9635844 | lifeskim:mentions | umls-concept:C0441889 | lld:lifeskim |
| pubmed-article:9635844 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:9635844 | lifeskim:mentions | umls-concept:C0806140 | lld:lifeskim |
| pubmed-article:9635844 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:9635844 | lifeskim:mentions | umls-concept:C0205225 | lld:lifeskim |
| pubmed-article:9635844 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:9635844 | pubmed:dateCreated | 1998-6-29 | lld:pubmed |
| pubmed-article:9635844 | pubmed:abstractText | The effect of 3-week, preoperative tamoxifen treatment on oestrogen receptor (ER) levels, expressed by primary breast tumours, was examined. Patients (age-matched) with breast cancer, confirmed by fine-needle aspiration, were either treated with 20 mg ml(-1) oral tamoxifen per day or received no medication in the 3-week interval between assessment and surgery. Quantification of ER using flow cytometry was performed on the surgically removed tumour samples from tamoxifen-treated (n = 40) and control (n = 38, untreated) patient groups. The tumours were mechanically disaggregated, and saponin treatment rendered these cells permeable to antibodies. Using dual-parameter labelling with a FITC-conjugated antibody (NCL-5D3) directed against cytokeratin 8/18/19 and a biotinylated antibody (DAKO-ER 1D5) directed against the oestrogen receptor, ER quantification was determined on a number of receptors per cell basis. Using QC quantum bead standards, ER levels in the epithelial cell population, the non-epithelial cell population and the whole-cell population (ER+) were calculated. ER levels were significantly lower in the total cell population than tamoxifen-treated patients (P = 0.002) when compared with the control (untreated) group. By using a gating procedure using 5D3 antibody positivity, a significantly lower level was detected on examining the cytokeratin-positive population alone (P = 0.006). Using a complementary gating technique, ER levels were quantified in the cytokeratin-negative cell population. Examination of this group of cells showed no significant difference between the levels of oestrogen receptor found in the tamoxifen-treated and untreated groups (P = 0.4). We have demonstrated that ER levels can be monitored by flow cytometry. ER levels in patients treated with tamoxifen 3 weeks before operation are significantly lower than in a comparative group of patients who received no drug. Furthermore, the most significant difference in receptor levels is seen by quantification of total ER levels expressed by all the tissue. | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9635844 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9635844 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9635844 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9635844 | pubmed:month | May | lld:pubmed |
| pubmed-article:9635844 | pubmed:issn | 0007-0920 | lld:pubmed |
| pubmed-article:9635844 | pubmed:author | pubmed-author:EganMM | lld:pubmed |
| pubmed-article:9635844 | pubmed:author | pubmed-author:CunliffeW JWJ | lld:pubmed |
| pubmed-article:9635844 | pubmed:author | pubmed-author:ShentonB KBK | lld:pubmed |
| pubmed-article:9635844 | pubmed:author | pubmed-author:HiggsM JMJ | lld:pubmed |
| pubmed-article:9635844 | pubmed:author | pubmed-author:YoungJ RJR | lld:pubmed |
| pubmed-article:9635844 | pubmed:author | pubmed-author:LuntL GLG | lld:pubmed |
| pubmed-article:9635844 | pubmed:author | pubmed-author:BrotherickII | lld:pubmed |
| pubmed-article:9635844 | pubmed:author | pubmed-author:WebbL ALA | lld:pubmed |
| pubmed-article:9635844 | pubmed:author | pubmed-author:BrowellD ADA | lld:pubmed |
| pubmed-article:9635844 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9635844 | pubmed:volume | 77 | lld:pubmed |
| pubmed-article:9635844 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9635844 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9635844 | pubmed:pagination | 1657-60 | lld:pubmed |
| pubmed-article:9635844 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:9635844 | pubmed:meshHeading | pubmed-meshheading:9635844-... | lld:pubmed |
| pubmed-article:9635844 | pubmed:meshHeading | pubmed-meshheading:9635844-... | lld:pubmed |
| pubmed-article:9635844 | pubmed:meshHeading | pubmed-meshheading:9635844-... | lld:pubmed |
| pubmed-article:9635844 | pubmed:meshHeading | pubmed-meshheading:9635844-... | lld:pubmed |
| pubmed-article:9635844 | pubmed:meshHeading | pubmed-meshheading:9635844-... | lld:pubmed |
| pubmed-article:9635844 | pubmed:meshHeading | pubmed-meshheading:9635844-... | lld:pubmed |
| pubmed-article:9635844 | pubmed:meshHeading | pubmed-meshheading:9635844-... | lld:pubmed |
| pubmed-article:9635844 | pubmed:meshHeading | pubmed-meshheading:9635844-... | lld:pubmed |
| pubmed-article:9635844 | pubmed:meshHeading | pubmed-meshheading:9635844-... | lld:pubmed |
| pubmed-article:9635844 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9635844 | pubmed:articleTitle | The effect of 3-week tamoxifen treatment on oestrogen receptor levels in primary breast tumours: a flow cytometric study. | lld:pubmed |
| pubmed-article:9635844 | pubmed:affiliation | Department of Surgery, University of Newcastle upon Tyne, UK. | lld:pubmed |
| pubmed-article:9635844 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9635844 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9635844 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9635844 | lld:pubmed |
