Linked Life Data

9635844

Source:http://linkedlifedata.com/resource/pubmed/id/9635844

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1998-6-29
pubmed:abstractText
The effect of 3-week, preoperative tamoxifen treatment on oestrogen receptor (ER) levels, expressed by primary breast tumours, was examined. Patients (age-matched) with breast cancer, confirmed by fine-needle aspiration, were either treated with 20 mg ml(-1) oral tamoxifen per day or received no medication in the 3-week interval between assessment and surgery. Quantification of ER using flow cytometry was performed on the surgically removed tumour samples from tamoxifen-treated (n = 40) and control (n = 38, untreated) patient groups. The tumours were mechanically disaggregated, and saponin treatment rendered these cells permeable to antibodies. Using dual-parameter labelling with a FITC-conjugated antibody (NCL-5D3) directed against cytokeratin 8/18/19 and a biotinylated antibody (DAKO-ER 1D5) directed against the oestrogen receptor, ER quantification was determined on a number of receptors per cell basis. Using QC quantum bead standards, ER levels in the epithelial cell population, the non-epithelial cell population and the whole-cell population (ER+) were calculated. ER levels were significantly lower in the total cell population than tamoxifen-treated patients (P = 0.002) when compared with the control (untreated) group. By using a gating procedure using 5D3 antibody positivity, a significantly lower level was detected on examining the cytokeratin-positive population alone (P = 0.006). Using a complementary gating technique, ER levels were quantified in the cytokeratin-negative cell population. Examination of this group of cells showed no significant difference between the levels of oestrogen receptor found in the tamoxifen-treated and untreated groups (P = 0.4). We have demonstrated that ER levels can be monitored by flow cytometry. ER levels in patients treated with tamoxifen 3 weeks before operation are significantly lower than in a comparative group of patients who received no drug. Furthermore, the most significant difference in receptor levels is seen by quantification of total ER levels expressed by all the tissue.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-10188917, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-1465279, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-1484969, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-183602, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-2448441, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-2463134, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-2919970, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-3084082, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-3690665, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-3965108, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-6142305, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-7214366, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-7297590, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-748014, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-7541332, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-7866090, http://linkedlifedata.com/resource/pubmed/commentcorrection/9635844-7924696
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0007-0920
pubmed:author
pubmed:issnType
Print
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1657-60
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
The effect of 3-week tamoxifen treatment on oestrogen receptor levels in primary breast tumours: a flow cytometric study.
pubmed:affiliation
Department of Surgery, University of Newcastle upon Tyne, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't