Linked Life Data

9635447

Source:http://linkedlifedata.com/resource/pubmed/id/9635447

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-6-25
pubmed:abstractText
The first part of this study of the biological mechanisms underlying attenuation of virulent Theileria annulata macroschizont-infected cell lines screened four pairs of T. annulata (Hisar) in vivo- and in vitro-derived macroschizont-infected cell lines (lines) and identified a single in vivo-derived line, which induced lethal tropical theileriosis. The other seven lines were relatively avirulent. Analysis of the clinical, hematological, and parasitological responses of cattle immunized with different passages of the virulent line after in vitro culture showed that it was partly attenuated by passage (p) 50 and avirulent by p130. Clones representing the three glucose phosphate isomerase (GPI) isotypes, which constituted the newly isolated virulent culture, were obtained from p3 by limiting dilution; p50 and p130 consisted of one isotype. The second part of the study raised monoclonal antibodies (MAbs) against macroschizont-infected cells, as reagents for detecting antigenic differences between virulent and avirulent parasites, and identified two MAbs that recognized the surface of infected cells as well as macroschizonts. MAb EU1 recognized an antigen expressed by all the lines tested, whether in vitro- or in vivo-derived, whether uncloned or cloned, and irrespective of extent of subpassage in culture. MAb EU106 recognized an antigen whose expression by the virulent line and its clones disappeared on passage in culture. This antigen was not expressed at all by the avirulent in vitro-derived line prepared with cells from the same calf. Both antigens were expressed by lines infected with other stocks of T. annulata, including two lines known to induce lethal disease. The different profiles of expression of the two novel antigens, recognized by MAbs EU1 and EU106, by the line undergoing attenuation suggest (1) that the two antigens interact differently with the bovine immune system; and (2) that there are two, very different, potential roles for these antibodies in the development of vaccines against T. annulata infections.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0014-4894
pubmed:author
pubmed:issnType
Print
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
228-40
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9635447-Animals, pubmed-meshheading:9635447-Antibodies, Monoclonal, pubmed-meshheading:9635447-Antigens, Protozoan, pubmed-meshheading:9635447-Antigens, Surface, pubmed-meshheading:9635447-Blood Cell Count, pubmed-meshheading:9635447-Cattle, pubmed-meshheading:9635447-Cell Line, pubmed-meshheading:9635447-Flow Cytometry, pubmed-meshheading:9635447-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:9635447-Gene Expression, pubmed-meshheading:9635447-Hematocrit, pubmed-meshheading:9635447-Isoenzymes, pubmed-meshheading:9635447-Leukocytes, Mononuclear, pubmed-meshheading:9635447-Protozoan Vaccines, pubmed-meshheading:9635447-Serial Passage, pubmed-meshheading:9635447-Theileria annulata, pubmed-meshheading:9635447-Theileriasis, pubmed-meshheading:9635447-Vaccines, Attenuated, pubmed-meshheading:9635447-Virulence
pubmed:year
1998
pubmed:articleTitle
Theileria annulata: the expression of two novel macroschizont antigens on the surface of infected mononuclear cells differs during in vitro attenuation of a virulent cell line.
pubmed:affiliation
Division of Biological Sciences, Institute of Cell, Animal and Population Biology, University of Edinburgh, Scotland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't