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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
26
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pubmed:dateCreated |
1998-8-3
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pubmed:abstractText |
To investigate a possible association between G-proteins and presenilin-1 (PS-1), a series of glutathione S-transferase-fusion proteins containing portions of PS-1 were prepared and used in vitro in binding experiments with tissue and recombinant G-proteins. The results demonstrate that the 39 C-terminal amino acids of PS-1 selectively bind the brain G-protein, Go. Addition of guanosine 5'-3-O-(thio)triphosphate promoted Go dissociation from PS-1, indicating that this domain mimics the function of G-protein-coupling domains found in receptors. The 39-amino acid synthetic polypeptide activated Go in a magnesium ion-dependent manner. Physical interaction of full-length PS-1 and Go was also demonstrated. Following transfection of Goalpha and N-terminally FLAG-tagged PS-1 in COS-7 cells, Go was immunoprecipitated by FLAG antibodies. In addition, endogenous PS-1 and Goalpha were colocalized immunocytochemically in human glioma cell lines. The results indicate that PS-1 regulates Go activities in living cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/FLAG peptide,
http://linkedlifedata.com/resource/pubmed/chemical/GTP Phosphohydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine 5'-O-(3-Thiotriphosphate),
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PSEN1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Presenilin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Wasp Venoms,
http://linkedlifedata.com/resource/pubmed/chemical/mastoparan
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:GAYJ PJP,
pubmed-author:GambettiPP,
pubmed-author:HomburgerVV,
pubmed-author:JogN VNV,
pubmed-author:KatadaTT,
pubmed-author:NishiyamaKK,
pubmed-author:OkamotoTT,
pubmed-author:ShiY CYC,
pubmed-author:SmineAA,
pubmed-author:YadavS PSP,
pubmed-author:YasuharaSS
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
16281-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9632688-Alzheimer Disease,
pubmed-meshheading:9632688-Amino Acid Sequence,
pubmed-meshheading:9632688-Animals,
pubmed-meshheading:9632688-Brain,
pubmed-meshheading:9632688-COS Cells,
pubmed-meshheading:9632688-Cells, Cultured,
pubmed-meshheading:9632688-Conserved Sequence,
pubmed-meshheading:9632688-Epitopes,
pubmed-meshheading:9632688-GTP Phosphohydrolases,
pubmed-meshheading:9632688-GTP-Binding Protein alpha Subunits, Gi-Go,
pubmed-meshheading:9632688-GTP-Binding Proteins,
pubmed-meshheading:9632688-Gene Expression Regulation,
pubmed-meshheading:9632688-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:9632688-Humans,
pubmed-meshheading:9632688-Membrane Proteins,
pubmed-meshheading:9632688-Molecular Sequence Data,
pubmed-meshheading:9632688-Peptides,
pubmed-meshheading:9632688-Presenilin-1,
pubmed-meshheading:9632688-Rats,
pubmed-meshheading:9632688-Recombinant Proteins,
pubmed-meshheading:9632688-Wasp Venoms
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pubmed:year |
1998
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pubmed:articleTitle |
Regulation of brain G-protein go by Alzheimer's disease gene presenilin-1.
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pubmed:affiliation |
The Lerner Research Institute, Department of Neurosciences, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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