Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
26
|
pubmed:dateCreated |
1998-8-3
|
pubmed:abstractText |
Hepatic stellate cells become activated during the early stages of hepatic injury associated with fibrogenesis. The mannose 6-phosphate/insulin-like growth factor-II receptor (M6P/IGFIIR) plays an important role in early fibrogenesis by participating in the activation of latent transforming growth factor-beta, a potent inducer of the matrix proteins in activated stellate cells that define the fibrotic phenotype. In this study we examined hepatic stellate cell regulation of M6P/IGFIIR expression and found that M6P/IGFIIR mRNA transcript levels increased in stellate cells from rats exposed to carbon tetrachloride (CCl4), a potent fibrogenic stimulant. Two E-boxes residing in the proximal promoter of M6P/IGFIIR were found to each bind a novel 75-kDa transcription factor (P75) in quiescent stellate cells of normal livers. This E-box binding was down-regulated as an early response in stellate cells exposed to CCl4, coinciding with increased M6P/IGFIIR transcript levels. Mutagenized E-boxes in M6P/IGFIIR promoter-chloramphenicol acetyltransferase (CAT) reporter constructs produced a substantial increase in reporter expression when compared with the corresponding native promoter-CAT construct when transfected in culture-activated stellate cells, suggesting P75's role as a repressor. The results indicate P75's participation in the regulation of M6P/IGFIIR transcription in hepatic stellate cells during fibrogenesis.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0021-9258
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
26
|
pubmed:volume |
273
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
15913-9
|
pubmed:dateRevised |
2008-11-21
|
pubmed:meshHeading |
pubmed-meshheading:9632637-Animals,
pubmed-meshheading:9632637-Carbon Tetrachloride Poisoning,
pubmed-meshheading:9632637-DNA,
pubmed-meshheading:9632637-Down-Regulation,
pubmed-meshheading:9632637-Liver,
pubmed-meshheading:9632637-Liver Cirrhosis, Experimental,
pubmed-meshheading:9632637-Male,
pubmed-meshheading:9632637-Molecular Weight,
pubmed-meshheading:9632637-Mutagenesis, Site-Directed,
pubmed-meshheading:9632637-Promoter Regions, Genetic,
pubmed-meshheading:9632637-RNA, Messenger,
pubmed-meshheading:9632637-Rats,
pubmed-meshheading:9632637-Rats, Sprague-Dawley,
pubmed-meshheading:9632637-Receptor, IGF Type 2,
pubmed-meshheading:9632637-Repressor Proteins,
pubmed-meshheading:9632637-Transfection,
pubmed-meshheading:9632637-Up-Regulation
|
pubmed:year |
1998
|
pubmed:articleTitle |
E-box-binding repressor is down-regulated in hepatic stellate cells during up-regulation of mannose 6-phosphate/insulin-like growth factor-II receptor expression in early hepatic fibrogenesis.
|
pubmed:affiliation |
Gastroenterology Section, Department of Medicine, University of Chicago Medical Center, Chicago, Illinois 60637, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|