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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1998-7-9
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pubmed:abstractText |
Broth culture supernatants from Tox+ Helicobacter pylori strains induce vacuolation of HeLa cells in vitro and contain VacA in concentrations that are higher than those found in supernatants from Tox- H. pylori strains. To investigate the basis for this phenomenon, we analyzed the transcription of the vacuolating cytotoxin gene (vacA) in eight Tox+ strains (each with a type s1/m1 vacA genotype) and nine Tox- strains (each with a type s2/m2 vacA genotype). Most of the Tox+ and Tox- strains tested used the same vacA transcriptional start point, but Tox+ strains yielded significantly stronger primer extension signal intensities than did Tox- strains (mean densitometry values of 15.8 +/- 1.9 versus 8.9 +/- 1.7, P = 0. 0016). Correspondingly, when we introduced vacA::xylE transcriptional fusions into the chromosomes of a Tox+ strain (60190) and a Tox- strain (86-313), the level of XylE activity in 60190 vacA::xylE was about 30-fold higher than that in 86-313 vacA::xylE. Sequence analysis and promoter exchange experiments indicated that the different levels of vacA transcription in these two strains cannot be explained solely by a difference in promoter strength. These data indicate that Tox+ and Tox- H. pylori strains typically differ not only in the VacA amino acid sequence but also in the level of vacA transcription.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-1370426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-1587837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-1695894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-2227449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-2449287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-3385767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-3584073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-6405380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-7629077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-7797934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-7854120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-7910603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-7937879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-8057855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-8144644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-8163943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-8168917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-8225576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-8666665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-8682800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-8733223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-8884364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-8890198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-8903587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9632570-9265644
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3088-94
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9632570-Artificial Gene Fusion,
pubmed-meshheading:9632570-Bacterial Proteins,
pubmed-meshheading:9632570-Bacterial Toxins,
pubmed-meshheading:9632570-Base Sequence,
pubmed-meshheading:9632570-Helicobacter pylori,
pubmed-meshheading:9632570-Molecular Sequence Data,
pubmed-meshheading:9632570-Phenotype,
pubmed-meshheading:9632570-Promoter Regions, Genetic,
pubmed-meshheading:9632570-Transcription, Genetic
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pubmed:year |
1998
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pubmed:articleTitle |
Heterogeneity in levels of vacuolating cytotoxin gene (vacA) transcription among Helicobacter pylori strains.
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pubmed:affiliation |
Departments of Medicine and Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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