|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0001511,
umls-concept:C0017262,
umls-concept:C0021755,
umls-concept:C0021758,
umls-concept:C0023516,
umls-concept:C0115305,
umls-concept:C0185117,
umls-concept:C0214743,
umls-concept:C0225336,
umls-concept:C1272706,
umls-concept:C2911684
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1998-7-15
|
|
pubmed:abstractText |
Leukocyte extravasation is governed by the endothelium, expressing a defined pattern of adhesion molecules in response to inflammatory stimuli. Among them, E-selectin, which is expressed in response to interleukin 1 (IL-1) or tumour necrosis factor alpha (TNF-alpha), provides rolling adhesion of the circulating leukocytes, a transient and reversible interaction that initiates leukocyte extravasation. In our experiments, E-selectin expression culminated after 4 to 6 h and declined thereafter. After 24 h a considerable amount of E-selectin was presented, reflecting its continuous expression in different inflammatory skin disorders. After preincubation of the endothelial cells with TNF-alpha together with IL-4 or IL-13, E-selectin mRNA transcription and protein expression were markedly reduced at 8 h and almost abolished at 20 h. In contrast, early E-selectin expression between 2 to 6 h was not significantly impaired. In a rotating adherence assay that mimics physiological shear forces in circulation, preincubation of the endothelial cells with TNF-alpha for 4 and 20 h induced similar adherence of neutrophils, which was largely E-selectin dependent. According to the modified expression kinetics of E-selectin in the presence of IL-4 of IL-13 rolling adhesion was unimpaired at 4 h but significantly diminished after 20 h of preincubation. Similar results were obtained with clones of the cutaneous T cell lymphoma cell line HUT78 highly expressing the E-selectin ligand cutaneous lymphocyte-associated Ag. Together, these data suggest that IL-4 and IL-13 control the rolling adhesion by limiting the period of the induced E-selectin expression and may thereby confine the acute phase of leukocyte emigration.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Blocking,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/CTAGE1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/E-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-13,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lymphocyte Homing,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Cell Adhesion Molecule-1
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
1043-4666
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright 1998 Academic Press Limited.
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
10
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
395-403
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:9632524-Antibodies, Blocking,
pubmed-meshheading:9632524-Antibodies, Monoclonal,
pubmed-meshheading:9632524-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:9632524-Antigens, Neoplasm,
pubmed-meshheading:9632524-Cell Adhesion,
pubmed-meshheading:9632524-Cells, Cultured,
pubmed-meshheading:9632524-Down-Regulation,
pubmed-meshheading:9632524-E-Selectin,
pubmed-meshheading:9632524-Endothelium, Vascular,
pubmed-meshheading:9632524-Flow Cytometry,
pubmed-meshheading:9632524-Humans,
pubmed-meshheading:9632524-Interleukin-1,
pubmed-meshheading:9632524-Interleukin-13,
pubmed-meshheading:9632524-Interleukin-4,
pubmed-meshheading:9632524-Membrane Glycoproteins,
pubmed-meshheading:9632524-Neutrophils,
pubmed-meshheading:9632524-Polymerase Chain Reaction,
pubmed-meshheading:9632524-Receptors, Lymphocyte Homing,
pubmed-meshheading:9632524-Time Factors,
pubmed-meshheading:9632524-Transcription, Genetic,
pubmed-meshheading:9632524-Tumor Cells, Cultured,
pubmed-meshheading:9632524-Tumor Necrosis Factor-alpha,
pubmed-meshheading:9632524-Vascular Cell Adhesion Molecule-1
|
|
pubmed:year |
1998
|
|
pubmed:articleTitle |
IL-4 and IL-13 downregulate rolling adhesion of leukocytes to IL-1 or TNF-alpha-activated endothelial cells by limiting the interval of E-selectin expression.
|
|
pubmed:affiliation |
Institute of Toxicology of the Swiss Federal Institute of Technology and University of Zurich, CH-8603 Schwerzenbach, Switzerland.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
