Linked Life Data

9630840

Source:http://linkedlifedata.com/resource/pubmed/id/9630840

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-7-1
pubmed:abstractText
The bursa of Fabricius represents the primary immune organ where immature B cells undergo maturational changes in avian species. Isolation of bursal lymphocytes for analysis in cell culture results in the rapid endogenous activation of apoptosis. After 2 h of incubation, over 45% of the lymphocytes were shown to be undergoing apoptosis and by 6 h 80% were undergoing apoptosis as demonstrated by a terminal deoxynucleotidyltransferase-mediated dUTP-fluorescein isothiocynate nick end-labeling flow-cytometric analysis. These results were corroborated by a propidium iodide-staining flow-cytometric assay and by an agarose gel electrophoresis DNA fragmentation assay that demonstrated internucleosomal DNA cleavage of genomic DNA in apoptotic bursal lymphocytes. Endogenous activation of apoptosis in bursal lymphocytes could be inhibited in a dose-dependent fashion with the phorbol ester, phorbol 12-myristate 13-acetate, but not the phorbol ester antagonist 4 alpha-phorbol 12,13-didecanoate. In addition, apoptosis could be inhibited in a dose-dependent fashion with inhibitors of protein translation, cycloheximide, and puromycin, as well as the transcriptional inhibitor actinomycin D. These results suggest that endogenous activation of bursal lymphocyte apoptosis may be mediated by the protein kinase C signal transduction pathway and activation of this process appears to be dependent upon de novo protein biosynthesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0008-8749
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
184
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
143-52
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Endogenous activation of apoptosis in bursal lymphocytes: inhibition by phorbol esters and protein synthesis inhibitors.
pubmed:affiliation
Department of Poultry Science, University of Georgia, Athens 30602-2772, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't