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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1998-7-9
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pubmed:abstractText |
We have previously demonstrated that interleukin 3 (IL-3) has a neurotrophic effect on central cholinergic neurons and have demonstrated the presence of IL-3 receptor (IL-3R)beta subunits in septal cholinergic neurons by reverse-transcribed polymerase chain reaction (RT-PCR) and immunohistochemistry. In order to confirm that the expressed IL-3R is functional, we conducted experiments to show an alpha subunit of IL-3R. The alpha subunit was clearly demonstrated by RT-PCR in the central cholinergic neuronal hybrid cell line SN6, but not in its mother cell line N18TG2, and the expression was slightly upregulated after IL-3 treatment. Choline acetyltransferase and vesicular acetylcholine transporter mRNAs were significantly increased in SN6 after treatment with IL-3. Immunohistochemically, IL-3R alpha-positive cells were mainly present in the medial septal and basal forebrain region, and the stained cells were similar to choline acetyltransferase-positive cells in shape and distribution. The IL-3R alpha-positive cells slightly increased two days after fimbria-fornix transection and decreased seven days after. These findings suggest that functional IL-3 receptors are expressed in the central cholinergic neurons and contribute to some physiological roles such as the differentiation and maintenance of these neurons.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0077-8923
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
840
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
107-16
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9629242-Animals,
pubmed-meshheading:9629242-Axotomy,
pubmed-meshheading:9629242-Blotting, Northern,
pubmed-meshheading:9629242-Brain,
pubmed-meshheading:9629242-Carrier Proteins,
pubmed-meshheading:9629242-Choline O-Acetyltransferase,
pubmed-meshheading:9629242-Cholinergic Fibers,
pubmed-meshheading:9629242-Immunohistochemistry,
pubmed-meshheading:9629242-Interleukin-3,
pubmed-meshheading:9629242-Male,
pubmed-meshheading:9629242-Membrane Transport Proteins,
pubmed-meshheading:9629242-Mice,
pubmed-meshheading:9629242-Mice, Inbred BALB C,
pubmed-meshheading:9629242-Neurons,
pubmed-meshheading:9629242-Polymerase Chain Reaction,
pubmed-meshheading:9629242-Receptors, Interleukin-3,
pubmed-meshheading:9629242-Staining and Labeling,
pubmed-meshheading:9629242-Transcription, Genetic,
pubmed-meshheading:9629242-Vesicular Acetylcholine Transport Proteins,
pubmed-meshheading:9629242-Vesicular Transport Proteins
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pubmed:year |
1998
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pubmed:articleTitle |
Interleukin-3 and interleukin-3 receptors in the brain.
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pubmed:affiliation |
Division of Demyelinating Disease and Aging, National Institute of Neuroscience, Tokyo, Japan. tabira@ncnaxp.ncnp.go.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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