| pubmed-article:962874 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:962874 | lifeskim:mentions | umls-concept:C0008051 | lld:lifeskim |
| pubmed-article:962874 | lifeskim:mentions | umls-concept:C0439849 | lld:lifeskim |
| pubmed-article:962874 | lifeskim:mentions | umls-concept:C0013707 | lld:lifeskim |
| pubmed-article:962874 | lifeskim:mentions | umls-concept:C0004434 | lld:lifeskim |
| pubmed-article:962874 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:962874 | lifeskim:mentions | umls-concept:C0053652 | lld:lifeskim |
| pubmed-article:962874 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:962874 | pubmed:dateCreated | 1976-10-29 | lld:pubmed |
| pubmed-article:962874 | pubmed:abstractText | 1. Biotin in chicken egg yolk is non-covalently bound to a specific protein that comprises 0.03% of the total yolk protein (0.8 mg/yolk). This biotin-binding protein is not detectable by the normal avidin assay owing to the biotin being tightly bound. Exchange of [14C]biotin for bound biotin at 65 degrees C is the basis of an assay for this protein. 2. Biotin-binding protein from egg yolk is distinguishable from egg-white avidin on Sephadex G-100 gel filtration, although the sizes of the two proteins appear quite similar. 3. Biotin-binding protein is denatured at a lower temperature and freely exchanges biotin at lower temperatures than does avidin. 4. The biotin-binding protein in egg yolk is postulated to be responsible for the deposition of biotin in egg yolk. D-[carboxyl-14C]Biotin injected into laying hens rapidly appears in the egg bound to yolk biotin-binding protein and avidin. Over 60% of the radioactivity is eventually deposited in eggs. The kinetics of biotin deposition in the egg suggests a 25 day half-life for an intracellular biotinyl-coenzyme pool in the laying hen. | lld:pubmed |
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| pubmed-article:962874 | pubmed:language | eng | lld:pubmed |
| pubmed-article:962874 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:962874 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:962874 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:962874 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:962874 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:962874 | pubmed:author | pubmed-author:McGuireJ CJC | lld:pubmed |
| pubmed-article:962874 | pubmed:author | pubmed-author:Della FeraM... | lld:pubmed |
| pubmed-article:962874 | pubmed:author | pubmed-author:WhiteH BHB3rd | lld:pubmed |
| pubmed-article:962874 | pubmed:author | pubmed-author:MeslarH WHW | lld:pubmed |
| pubmed-article:962874 | pubmed:author | pubmed-author:WhitneyC JCJ | lld:pubmed |
| pubmed-article:962874 | pubmed:author | pubmed-author:SammelwitzP... | lld:pubmed |
| pubmed-article:962874 | pubmed:author | pubmed-author:DennisonB ABA | lld:pubmed |
| pubmed-article:962874 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:962874 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:962874 | pubmed:volume | 157 | lld:pubmed |
| pubmed-article:962874 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:962874 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:962874 | pubmed:pagination | 395-400 | lld:pubmed |
| pubmed-article:962874 | pubmed:dateRevised | 2010-9-3 | lld:pubmed |
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| pubmed-article:962874 | pubmed:meshHeading | pubmed-meshheading:962874-A... | lld:pubmed |
| pubmed-article:962874 | pubmed:year | 1976 | lld:pubmed |
| pubmed-article:962874 | pubmed:articleTitle | Biotin-binding protein from chicken egg yolk. Assay and relationship to egg-white avidin. | lld:pubmed |
| pubmed-article:962874 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:962874 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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