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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1976-10-29
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pubmed:abstractText |
1. Biotin in chicken egg yolk is non-covalently bound to a specific protein that comprises 0.03% of the total yolk protein (0.8 mg/yolk). This biotin-binding protein is not detectable by the normal avidin assay owing to the biotin being tightly bound. Exchange of [14C]biotin for bound biotin at 65 degrees C is the basis of an assay for this protein. 2. Biotin-binding protein from egg yolk is distinguishable from egg-white avidin on Sephadex G-100 gel filtration, although the sizes of the two proteins appear quite similar. 3. Biotin-binding protein is denatured at a lower temperature and freely exchanges biotin at lower temperatures than does avidin. 4. The biotin-binding protein in egg yolk is postulated to be responsible for the deposition of biotin in egg yolk. D-[carboxyl-14C]Biotin injected into laying hens rapidly appears in the egg bound to yolk biotin-binding protein and avidin. Over 60% of the radioactivity is eventually deposited in eggs. The kinetics of biotin deposition in the egg suggests a 25 day half-life for an intracellular biotinyl-coenzyme pool in the laying hen.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/962874-14007179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/962874-14040270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/962874-17783793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/962874-237414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/962874-237761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/962874-4293925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/962874-4594672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/962874-4643132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/962874-4729821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/962874-5436593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/962874-5480019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/962874-5645506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/962874-6053648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/962874-805200
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
157
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
395-400
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pubmed:dateRevised |
2010-9-3
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pubmed:meshHeading |
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pubmed:year |
1976
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pubmed:articleTitle |
Biotin-binding protein from chicken egg yolk. Assay and relationship to egg-white avidin.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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