Linked Life Data

9628577

Source:http://linkedlifedata.com/resource/pubmed/id/9628577

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-8-25
pubmed:abstractText
Plasmid pKYM, isolated from the gram-negative bacterium Shigella sonnei, is a small multicopy plasmid which replicates by a rolling circle mechanism. The formation of multimers has been observed in a derivative of pKYM which lost a part of the origin region, and the loss of the monomerization mechanism would have led to these multimers. By analyzing the constructs of several mutants, we discovered that a DNA region required for monomerization was present upstream of the RepK binding site in the replication origin. As either of the T-rich sequence or the inverted repeat sequences which were seen in that region have been lost in the multimer-forming plasmids, these sequences may be necessary for monomerization.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1340-2838
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11-4
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Identification of the region required for monomerization of the rolling circle plasmid pKYM.
pubmed:affiliation
Division of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Japan.
pubmed:publicationType
Journal Article