Linked Life Data

9628367

Source:http://linkedlifedata.com/resource/pubmed/id/9628367

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4-5
pubmed:dateCreated
1998-8-11
pubmed:abstractText
Type III restriction endonucleases recognize nonsymmetric nucleotide sequences. A necessary condition for DNA cleavage is the presence of two unmethylated recognition sites which are inversely ('head-to-head') oriented in the DNA double strand. A DNA substrate possessing one EcoP1 and one EcoP15 site in the head-to-head configuration could not be cleaved by the individual enzymes, however, it was specifically digested in the simultaneous presence of both enzymes. In agreement with the tracking-collision model for the DNA interaction of type III enzymes cleavage could be abolished by Lac repressor bound between the two sites. We conclude that two different type III enzymes can functionally cooperate in the cleavage of DNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1431-6730
pubmed:author
pubmed:issnType
Print
pubmed:volume
379
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
617-20
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Mutual activation of two restriction endonucleases: interaction of EcoP1 and EcoP15.
pubmed:affiliation
Institute of Medical Virology, Charité Medical School, Humboldt University, Berlin, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't