Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-9-1
pubmed:abstractText
Bovine alpha s1-casein F (alpha s1-CN F) was found in a genetic resource of Deutsches Schwarzbuntes Niederungsrind cows at a frequency of 0.009. Biochemical characterization of this new variant was obtained by automated sequencing of reversed-phase HPLC-separated tryptic peptides of alpha s1-CN F and alpha s1-CN B. alpha s1-CN F was found to be a subtype of alpha s1-CN B with a single amino acid substitution (SerP/Leu) in position 66. DNA sequencing revealed a C/T transition in position 8418 of the gene. Sequence-specific primers were designed to perform an allele-specific polymerase chain reaction for detection of alpha s1 CnF. Typing of artificial insemination sperm samples included in the genetic resource sperm pool identified one sire heterozygous for alpha s1 CnF.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-0299
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
223-31
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9627841-Alleles, pubmed-meshheading:9627841-Amino Acid Sequence, pubmed-meshheading:9627841-Animals, pubmed-meshheading:9627841-Caseins, pubmed-meshheading:9627841-Cattle, pubmed-meshheading:9627841-Chromatography, High Pressure Liquid, pubmed-meshheading:9627841-Cytosine, pubmed-meshheading:9627841-DNA Primers, pubmed-meshheading:9627841-Evolution, Molecular, pubmed-meshheading:9627841-Female, pubmed-meshheading:9627841-Genetic Variation, pubmed-meshheading:9627841-Genotype, pubmed-meshheading:9627841-Milk, pubmed-meshheading:9627841-Molecular Sequence Data, pubmed-meshheading:9627841-Peptide Fragments, pubmed-meshheading:9627841-Point Mutation, pubmed-meshheading:9627841-Polymerase Chain Reaction, pubmed-meshheading:9627841-Recombinant Proteins, pubmed-meshheading:9627841-Sequence Deletion, pubmed-meshheading:9627841-Thymine, pubmed-meshheading:9627841-Trypsin
pubmed:year
1998
pubmed:articleTitle
Biochemical characterization of bovine alpha s1-casein F and genotyping with sequence-specific primers.
pubmed:affiliation
Institut fur Tierzucht und Haustiergenetik, Justus-Liebig-Universität Giessen, Deutschland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't