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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0019627,
umls-concept:C0024348,
umls-concept:C0205148,
umls-concept:C0205314,
umls-concept:C0431085,
umls-concept:C0567416,
umls-concept:C0597032,
umls-concept:C0679622,
umls-concept:C1171362,
umls-concept:C1314939,
umls-concept:C1416942,
umls-concept:C1515670,
umls-concept:C1515881
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pubmed:issue |
12
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pubmed:dateCreated |
1998-7-7
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pubmed:abstractText |
After culture in interleukin (IL)-2, natural killer (NK) cells acquire an increased capability of mediating non-major histocompatibility complex (MHC)-restricted tumor cell lysis. This may reflect, at least in part, the de novo expression by NK cells of triggering receptors involved in cytolysis. In this study we identified a novel 44-kD surface molecule (NKp44) that is absent in freshly isolated peripheral blood lymphocytes but is progressively expressed by all NK cells in vitro after culture in IL-2. Different from other markers of cell activation such as CD69 or VLA.2, NKp44 is absent in activated T lymphocytes or T cell clones. Since NKp44 was not detected in any of the other cell lineages analyzed, it appears as the first marker specific for activated human NK cells. Monoclonal antibody (mAb)-mediated cross-linking of NKp44 in cloned NK cells resulted in strong activation of target cell lysis in a redirected killing assay. This data indicated that NKp44 can mediate triggering of NK cell cytotoxicity. mAb-mediated masking of NKp44 resulted in partial inhibition of cytolytic activity against certain (FcgammaR-negative) NK-susceptible target cells. This inhibition was greatly increased by the simultaneous masking of p46, another recently identified NK-specific triggering surface molecule. These data strongly suggest that NKp44 functions as a triggering receptor selectively expressed by activated NK cells that, together with p46, may be involved in the process of non-MHC-restricted lysis. Finally, we show that p46 and NKp44 are coupled to the intracytoplasmic transduction machinery via the association with CD3zeta or KARAP/DAP12, respectively; these associated molecules are tyrosine phosphorylated upon NK cell stimulation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-1720808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-1836482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-1845873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-1946452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-2137855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-2188667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-2532305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-2683611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-2809220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-2903209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-3100633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-7650491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-8149950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-8627176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-8717527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-8765026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-9057360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-9059886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-9059890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-9059891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-9133416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-9164921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-9182676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-9314561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-9430220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9625766-9490415
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
187
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2065-72
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9625766-Biological Markers,
pubmed-meshheading:9625766-Cells, Cultured,
pubmed-meshheading:9625766-Cytotoxicity, Immunologic,
pubmed-meshheading:9625766-Flow Cytometry,
pubmed-meshheading:9625766-Humans,
pubmed-meshheading:9625766-Interleukin-2,
pubmed-meshheading:9625766-Killer Cells, Natural,
pubmed-meshheading:9625766-Minor Histocompatibility Antigens,
pubmed-meshheading:9625766-Natural Cytotoxicity Triggering Receptor 1,
pubmed-meshheading:9625766-Natural Cytotoxicity Triggering Receptor 2,
pubmed-meshheading:9625766-Receptors, Immunologic,
pubmed-meshheading:9625766-Signal Transduction
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pubmed:year |
1998
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pubmed:articleTitle |
NKp44, a novel triggering surface molecule specifically expressed by activated natural killer cells, is involved in non-major histocompatibility complex-restricted tumor cell lysis.
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pubmed:affiliation |
Istituto Nazionale per la Ricerca sul Cancro and Centro Biotecnologie Avanzate, 16132 Genova, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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