Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1998-9-3
|
| pubmed:abstractText |
The effect of HHV-6 strain A infection on the expression of Epstein-Barr virus- (EBV-) encoded growth transformation-associated genes in two EBV-positive Burkitt lymphoma cell lines, Akata and P3HR-3, was investigated. The results indicate that HHV-6A upregulates the expression of the latent membrane protein LMP-1 in both cell lines. Expression of EBNA-2 was also upregulated in Akata cells following HHV-6A infection. Transfection of reporter constructs carrying the LMP-1 regulatory sequences (LRS; -634/+40) or its 5' deleted derivatives in Akata and in a T-lymphoblastoid cell line, J-Jhan, confirmed the presence of positive and negative regulatory elements responsive to HHV-6A infection in LMP-1 regulatory sequence (LRS). The majority of LRS constructs were under the influence of dominant negative factors. HHV-6A was able to override the effect of such factors acting on reporter plasmids containing the -634/-54, -324/-54, -214/-54, and -106/-54 parts of LRS. The plasmid that carried only the -54/+40 LRS region was constitutively active in both Akata and J-Jhan cells; in Akata, its activity was influenced by HHV-6A. The finding that HHV-6A infection may activate LMP-1 and EBNA-2 expression, which is essential for the immortalization of B-lymphocytes by EBV, shows a novel aspect of the interaction between these two herpesviruses.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/EBV-associated membrane antigen...,
http://linkedlifedata.com/resource/pubmed/chemical/Epstein-Barr Virus Nuclear Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphonoacetic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Matrix Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0146-6615
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
55
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
219-26
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9624610-Antibodies, Monoclonal,
pubmed-meshheading:9624610-Blotting, Western,
pubmed-meshheading:9624610-Burkitt Lymphoma,
pubmed-meshheading:9624610-Epstein-Barr Virus Nuclear Antigens,
pubmed-meshheading:9624610-Fluorescent Antibody Technique,
pubmed-meshheading:9624610-Gene Expression Regulation, Viral,
pubmed-meshheading:9624610-Genes, Viral,
pubmed-meshheading:9624610-Herpesviridae Infections,
pubmed-meshheading:9624610-Herpesvirus 4, Human,
pubmed-meshheading:9624610-Herpesvirus 6, Human,
pubmed-meshheading:9624610-Humans,
pubmed-meshheading:9624610-Phosphonoacetic Acid,
pubmed-meshheading:9624610-Plasmids,
pubmed-meshheading:9624610-Promoter Regions, Genetic,
pubmed-meshheading:9624610-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:9624610-Superinfection,
pubmed-meshheading:9624610-Tumor Cells, Cultured,
pubmed-meshheading:9624610-Up-Regulation,
pubmed-meshheading:9624610-Viral Matrix Proteins,
pubmed-meshheading:9624610-Virus Latency
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Upregulation of Epstein-Barr virus-encoded latent membrane protein by human herpesvirus 6 superinfection of EBV-carrying Burkitt lymphoma cells.
|
| pubmed:affiliation |
Istituto Neurologico Mediterraneo Neuromed, Pozzilli, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|