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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1998-7-14
|
| pubmed:abstractText |
A capillary array electrophoresis apparatus capable of running and analyzing 48 DNA sequencing samples simultaneously has been constructed. The instrument uses a replaceable sieving buffer and incorporates a convenient method for introducing the buffer into the capillaries. Data from laser-induced fluorescence are collected as four separate images, one for each optical channel. The integrated data analysis software employs an open architecture that allows use of any DNA base-calling algorithm. DNA sequencing runs are completed in approx. 1 hr (approximately 500 bases), and instrument turnaround time between runs is less than 15 min. Overall, the instrument throughput is on the order of 720 templates/day, or 360,000 bases/day.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
1079-5383
|
| pubmed:author |
pubmed-author:BarkerDD,
pubmed-author:BashkinJJ,
pubmed-author:BechtolKK,
pubmed-author:CartwrightPP,
pubmed-author:DolnikVV,
pubmed-author:HarrisDD,
pubmed-author:LULL,
pubmed-author:MarksAA,
pubmed-author:MarshMM,
pubmed-author:RoachDD,
pubmed-author:ShiX BXB,
pubmed-author:SmietanaPP,
pubmed-author:SolomonNN,
pubmed-author:WangLL
|
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
83-9
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading | |
| pubmed:articleTitle |
High-throughput DNA sequencing on a capillary array electrophoresis system.
|
| pubmed:affiliation |
Molecular Dynamics, Sunnyvale, CA, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|