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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1998-8-27
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pubmed:abstractText |
We examined the relative importance of G (Gi) protein-coupled brain-type (CB1-R) and spleen-type (CB2-R) cannabinoid receptors in preimplantation embryo development using agonists and antagonists specific to CB1-R and CB2-R. The results establish that endogenous cannabinoid ligands, anandamide and sn-2 arachidonoylglycerol, arrest embryo development in vitro, and this effect is reversed by CB1-R antagonists SR141716A or AM 251, but not by SR144528, a CB2-R antagonist. A CB2-R selective agonist AM 663 failed to affect embryo development. These results suggest that cannabinoid effects on embryo development are mediated by CB1-R. We also observed that delta9-tetrahydrocannabinol ([-]THC) infused in the presence of cytochrome P450 inhibitors interfered with blastocyst implantation. This adverse effect was reversed by coinfusion of SR141716A. The less active stereoisomer (+)THC plus the inhibitors failed to affect implantation. Analysis of tissue levels demonstrated that uterine accumulation of (-)THC occurred when it was infused in the presence of the P450 inhibitors. These results demonstrate that the uterus and perhaps the embryo have the cytochrome P450 enzymes to metabolize (-)THC and neutralize its adverse effects on implantation. Collectively, the present study demonstrates that cannabinoid effects on embryo development and implantation are mediated by embryonic and/or uterine CB1-R, but not CB2-R.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-arachidonylglycerol,
http://linkedlifedata.com/resource/pubmed/chemical/AM 251,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Cannabinoids,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Glycerides,
http://linkedlifedata.com/resource/pubmed/chemical/Piperidines,
http://linkedlifedata.com/resource/pubmed/chemical/Polyunsaturated Alkamides,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cannabinoid,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Drug,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrahydrocannabinol,
http://linkedlifedata.com/resource/pubmed/chemical/anandamide,
http://linkedlifedata.com/resource/pubmed/chemical/rimonabant
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-3363
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
58
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1490-5
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9623610-Animals,
pubmed-meshheading:9623610-Arachidonic Acids,
pubmed-meshheading:9623610-Brain,
pubmed-meshheading:9623610-Cannabinoids,
pubmed-meshheading:9623610-Cytochrome P-450 Enzyme System,
pubmed-meshheading:9623610-Embryo, Mammalian,
pubmed-meshheading:9623610-Embryo Implantation,
pubmed-meshheading:9623610-Embryonic Development,
pubmed-meshheading:9623610-Embryonic and Fetal Development,
pubmed-meshheading:9623610-Enzyme Inhibitors,
pubmed-meshheading:9623610-Female,
pubmed-meshheading:9623610-Glycerides,
pubmed-meshheading:9623610-Male,
pubmed-meshheading:9623610-Mice,
pubmed-meshheading:9623610-Piperidines,
pubmed-meshheading:9623610-Polyunsaturated Alkamides,
pubmed-meshheading:9623610-Pregnancy,
pubmed-meshheading:9623610-Pyrazoles,
pubmed-meshheading:9623610-Receptors, Cannabinoid,
pubmed-meshheading:9623610-Receptors, Drug,
pubmed-meshheading:9623610-Tetrahydrocannabinol
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pubmed:year |
1998
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pubmed:articleTitle |
Effects of cannabinoids on preimplantation mouse embryo development and implantation are mediated by brain-type cannabinoid receptors.
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pubmed:affiliation |
Department of Molecular and Integrative Physiology, Ralph L. Smith Research Center, University of Kansas Medical Center, Kansas City 66160-7338, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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