Linked Life Data

9623592

Source:http://linkedlifedata.com/resource/pubmed/id/9623592

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1998-8-27
pubmed:abstractText
Retinoids have previously been shown to be crucial for normal spermatogenesis. The role of retinoic acid receptors has been studied, but relatively little is known about the function of retinoid X receptors (RXRs). To gain more insight in the function of RXRs during spermatogenesis, the cellular localization of RXRs in the mouse testis was examined using immunohistochemistry and RNase protection assays. In both normal and vitamin A-deficient (VAD) testes, a strong immune response to an RXRalpha antibody occurred in Leydig cells, peritubular myoid cells, and A spermatogonia. Weaker signals were found in spermatocytes and spermatids. In normal testes, an RXRbeta antibody gave a reaction in Leydig cells, and, to a lesser extent, in Sertoli cells, A spermatogonia, pachytene spermatocytes, and spermatids. In Leydig cells, a cytoplasmatic signal was found in addition to the nuclear signal. In the VAD testis, only Leydig cells and A spermatogonia were positive, which indicates that RXRbeta expression may be dependent on the retinoid status. Previous studies have shown RXRgamma mRNA expression in the mouse testis at a low level. Nevertheless, an RXRgamma antibody caused a strong immune response in interstitial cells and in A spermatogonia, and a weak signal in pachytene spermatocytes. These immunohistochemical data were supported by the results of RNase protection assays on mRNA of testicular cell isolations. In conclusion, the different RXRs in the mouse testis have distinct expression patterns, suggesting that they may have different functions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1351-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Differential expression pattern of retinoid X receptors in adult murine testicular cells implies varying roles for these receptors in spermatogenesis.
pubmed:affiliation
Department of Cell Biology, Utrecht University, The Netherlands. gaemers@nki.nl
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't