Linked Life Data

9622084

Source:http://linkedlifedata.com/resource/pubmed/id/9622084

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1998-7-7
pubmed:abstractText
Administration of recombinant interleukin 12 (IL-12) induces tumor regression that is associated with T-cell infiltration in the OV-HM ovarian carcinoma and CSA1M fibrosarcoma models. After confirming the blocking of regression by injection of anti-IFN-gamma monoclonal antibody (mAb), we investigated the mechanisms underlying the requirement of IFN-gamma in T-cell migration and tumor regression. T-cell migration was inhibited by injection of anti-IFN-gamma mAb to OV-HM tumor-bearing mice prior to IL-12 treatment. We examined, using the lymphoid cell migration assay, whether IFN-gamma is required for enhancing the migratory capacity of T cells or the T cell-accepting potential of tumor masses during IL-12 treatment. Spleen cells from IL-12-treated or untreated OV-HM-bearing mice were stained in vitro with a fluorescein chemical and transferred i.v. into OV-HM-bearing mice that were not treated with IL-12. Migration of donor cells was quantitated by counting the number of fluorescent cells on cryostat sections of tumor masses from recipient mice. Compared to spleen cells from OV-HM-bearing mice that were not treated with IL-12, enhanced migration was observed for cells from IL-12-treated OV-HM-bearing mice. Anti-IFN-gamma pretreatment of donor mice before IL-12 treatment did not reduce the migratory capacity of T cells, whereas migration was markedly inhibited in recipient mice injected with anti-IFN-gamma. Anti-IFN-gamma pretreatment decreased vascular cell adhesion molecule-1 (VCAM-1)-/intercellular adhesion molecule-1 (ICAM-1)-positive blood vessels at tumor sites. Consistent with this, migration was also inhibited by treatment of recipient mice with either anti-VCAM-1 or anti-ICAM-1 mAb. In contrast to the OV-HM model, T-cell migration was not affected in the CSA1M model following preinjection of anti-IFN-gamma mAb. In this model, VCAM-1-/ICAM-1-positive blood vessels existed even after anti-IFN-gamma treatment, although tumor regression was completely inhibited. These results indicate that IFN-gamma plays two distinct roles in expressing the antitumor efficacy of IL-12: one is to support the T-cell acceptability of tumor masses, and the other is to mediate the antitumor effects of migrated T cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2426-32
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9622084-Animals, pubmed-meshheading:9622084-Antibodies, Monoclonal, pubmed-meshheading:9622084-Antineoplastic Agents, pubmed-meshheading:9622084-Cell Movement, pubmed-meshheading:9622084-Down-Regulation, pubmed-meshheading:9622084-Female, pubmed-meshheading:9622084-Fibrosarcoma, pubmed-meshheading:9622084-Intercellular Adhesion Molecule-1, pubmed-meshheading:9622084-Interferon-gamma, pubmed-meshheading:9622084-Interleukin-12, pubmed-meshheading:9622084-Male, pubmed-meshheading:9622084-Mice, pubmed-meshheading:9622084-Mice, Inbred BALB C, pubmed-meshheading:9622084-Mice, Inbred C57BL, pubmed-meshheading:9622084-Neoplasms, Experimental, pubmed-meshheading:9622084-Neovascularization, Pathologic, pubmed-meshheading:9622084-Ovarian Neoplasms, pubmed-meshheading:9622084-Rats, pubmed-meshheading:9622084-Remission Induction, pubmed-meshheading:9622084-T-Lymphocytes, pubmed-meshheading:9622084-Tumor Cells, Cultured, pubmed-meshheading:9622084-Vascular Cell Adhesion Molecule-1
pubmed:year
1998
pubmed:articleTitle
Multiple roles of interferon-gamma in the mediation of interleukin 12-induced tumor regression.
pubmed:affiliation
Biomedical Research Center, Osaka University Medical School, Suita, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't