Linked Life Data

9621286

Source:http://linkedlifedata.com/resource/pubmed/id/9621286

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1998-8-24
pubmed:abstractText
Vascular endothelial growth factor (VEGF) has an important function in renal vascular ontogenesis and is constitutively expressed in podocytes of the adult kidney. The ability of VEGF to be chemotactic for monocytes and to increase the activity of collagenase and plasminogen activator may have implications for renal development and renal disease. In humans, the cellular actions of VEGF depend on binding to two specific receptors: Flt-1 and KDR. The aims of this study were: (1) to localize VEGF receptor proteins in human renal ontogenesis; (2) to quantify VEGF binding in human fetal and adult kidney; and (3) to dissect the binding into its two known components: the KDR and Flt-1 receptors. The latter aim was achieved by competitive binding of VEGF and placenta growth factor-2, which only binds to Flt-1. Quantification of 125I-VEGF binding sites was performed by autoradiography and computerized densitometry. By double-label immunohistochemistry, VEGF receptor proteins were localized solely to endothelial cells of preglomerular vessels, glomeruli, and postglomerular vessels. In developing glomeruli, VEGF receptor protein appeared as soon as endothelial cells were positive for von Willebrand factor. Specific 125I-VEGF binding could be localized to renal arteries and veins, glomeruli, and the tubulointerstitial capillary network in different developmental stages. Affinity (Kd) of adult (aK) and fetal (fK) kidneys was: Kd: glomeruli 38.6 +/- 11.2 (aK, n = 5), 36.3 +/- 7.1 (fK, n = 5); cortical tubulointerstitium 19.4 +/- 2.6 (aK, n = 5), 11.6 +/- 7.0 (fK, n = 5) pmol. Placenta growth factor-2 displaced VEGF binding in all renal structures by approximately 60%. VEGF receptor proteins thus were found only in renal endothelial cells. A coexpression of both VEGF binding sites could be shown, with Flt-1 demonstrating the most abundant VEGF receptor binding sites in the kidney. These studies support the hypothesis of a function for VEGF in adult kidney that is independent of angiogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1046-6673
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1032-44
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9621286-Adult, pubmed-meshheading:9621286-Aging, pubmed-meshheading:9621286-Animals, pubmed-meshheading:9621286-Binding Sites, pubmed-meshheading:9621286-Female, pubmed-meshheading:9621286-Fetus, pubmed-meshheading:9621286-Fluorescent Antibody Technique, pubmed-meshheading:9621286-Humans, pubmed-meshheading:9621286-Immunohistochemistry, pubmed-meshheading:9621286-Iodine Radioisotopes, pubmed-meshheading:9621286-Kidney, pubmed-meshheading:9621286-Male, pubmed-meshheading:9621286-Mice, pubmed-meshheading:9621286-Mice, Inbred BALB C, pubmed-meshheading:9621286-Middle Aged, pubmed-meshheading:9621286-Rabbits, pubmed-meshheading:9621286-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:9621286-Receptors, Growth Factor, pubmed-meshheading:9621286-Receptors, Vascular Endothelial Growth Factor, pubmed-meshheading:9621286-Swine, pubmed-meshheading:9621286-Tissue Distribution
pubmed:year
1998
pubmed:articleTitle
Receptors of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) in fetal and adult human kidney: localization and [125I]VEGF binding sites.
pubmed:affiliation
Institute of Pathology, Philipps University, Marburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't