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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1998-7-1
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pubmed:abstractText |
The epidemiology of human immunodeficiency virus (HIV)-associated Kaposi's sarcoma (KS) resembles that of a sexually transmitted pathogen. However, human herpesvirus 8 (HHV-8), the proposed cause of KS, is found in semen only infrequently and at low titers. To determine whether HHV-8 was present in the urogenital tract, transrectal ultrasound-guided prostate biopsies were obtained from six men with KS (five with concurrent HIV infection) and four without KS (three with concurrent HIV) and assayed for HHV-8 by PCR. Nine of the 10 men were seropositive for HHV-8. Five of nine HHV-8-seropositive men had HHV-8 DNA detected in prostate tissue by solution-based PCR. All five currently had KS or had it previously. In two subjects, prostate tissue was the only identified source of HHV-8. In situ PCR on serial sections of prostate indicated that HHV-8 infection was localized to discrete areas of the prostate. When detected, HHV-8 DNA was present in the nuclei of >90% of the glandular epithelial cells. In situ hybridization for HHV-8 mRNA revealed that between 1 and 5% of cells harboring HHV-8 DNA expressed viral transcripts associated with HHV-8 replication (T1.1 transcript), while >90% expressed gene products associated with viral latency (T0.7 transcript). Intermittent replication of HHV-8 in the prostate and subsequent shedding of virus in semen may be crucial factors for determining whether HHV-8 can be transmitted through sexual activity.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-7700310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-8318169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-8602183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-8612236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-8692871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-8705863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-8744575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-8985403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-8995643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-9030373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-9030389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-9207353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-9207354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-9261440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-9291331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9621094-9557671
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6223-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9621094-Adult,
pubmed-meshheading:9621094-Aged,
pubmed-meshheading:9621094-DNA, Viral,
pubmed-meshheading:9621094-Herpesvirus 8, Human,
pubmed-meshheading:9621094-Humans,
pubmed-meshheading:9621094-Male,
pubmed-meshheading:9621094-Middle Aged,
pubmed-meshheading:9621094-Polymerase Chain Reaction,
pubmed-meshheading:9621094-Prostate,
pubmed-meshheading:9621094-Sarcoma, Kaposi
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pubmed:year |
1998
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pubmed:articleTitle |
Human herpesvirus 8 in the prostate glands of men with Kaposi's sarcoma.
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pubmed:affiliation |
Department of Medicine, and Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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