Linked Life Data

9620406

Source:http://linkedlifedata.com/resource/pubmed/id/9620406

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1998-7-28
pubmed:abstractText
The sensitivities and specificities of 17 antibody detection tests for brucellosis in goats were estimated. Tests evaluated included the U.S. Department of Agriculture (USDA) card test with 8% cell concentration (8%Card), USDA rapid automated presumptive test (RAP), Mexican rose bengal plate tests with 8 and 3% cell concentrations (8%RB and 3%RB), French rose bengal plate test with 4.5% cell concentration (4.5%RB), USDA standard plate test (SPT), USDA buffered acidified plate agglutination test (BAPA), USDA and Mexican rivanol tests (URIV and MRIV), USDA standard tube tests with Brucella abortus and Brucella melitensis antigens (SATA and SATM), serum enzyme-linked immunosorbent assay (ELISA), USDA cold-fixation complement fixation tests with B. abortus and B. melitensis antigens (CFA and CFM), USDA and Mexican milk ring tests (UBRT and MBRT), and a milk ELISA. Test sensitivity was evaluated by using two groups of 10 goats experimentally infected with B. melitensis or B. abortus and monitored for 24 weeks. Specificity was evaluated by using 200 brucellosis-free nonvaccinated goats from 10 California herds. The 3%RB was considered a good screening test because of high sensitivity at week 24 postinfection (90%), ease of performance, and low cost. The cold-fixation CFA and CFM had 100% specificity in the field study and were considered appropriate confirmatory tests. The milk ELISA was significantly more sensitive (P < 0.05) than the UBRT and significantly more specific (P < 0.05) than the MBRT. The milk ELISA also had the advantage of objectivity and ease of interpretation.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1716-22
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:9620406-Agglutination Tests, pubmed-meshheading:9620406-Animals, pubmed-meshheading:9620406-Antibodies, Bacterial, pubmed-meshheading:9620406-Antigens, Bacterial, pubmed-meshheading:9620406-Brucella abortus, pubmed-meshheading:9620406-Brucella melitensis, pubmed-meshheading:9620406-Brucellosis, pubmed-meshheading:9620406-Complement Fixation Tests, pubmed-meshheading:9620406-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:9620406-Evaluation Studies as Topic, pubmed-meshheading:9620406-Female, pubmed-meshheading:9620406-Goat Diseases, pubmed-meshheading:9620406-Goats, pubmed-meshheading:9620406-Male, pubmed-meshheading:9620406-Milk, pubmed-meshheading:9620406-Sensitivity and Specificity, pubmed-meshheading:9620406-Serologic Tests, pubmed-meshheading:9620406-United States, pubmed-meshheading:9620406-United States Department of Agriculture
pubmed:year
1998
pubmed:articleTitle
Evaluation of North American antibody detection tests for diagnosis of brucellosis in goats.
pubmed:affiliation
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis 95616, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't