rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
1
|
pubmed:dateCreated |
1998-6-18
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pubmed:databankReference |
|
pubmed:abstractText |
The Na+-H+ exchanger regulatory factor (NHE-RF) is a cytoplasmic phosphoprotein that was first found to be involved in protein kinase A mediated regulation of ion transport. NHE-RF contains two distinct protein interaction PDZ domains: NHE-RF-PDZ1 and NHE-RF-PDZ2. However, their binding partners are currently unknown. Because PDZ domains usually bind to specific short linear C-terminal sequences, we have carried out affinity selection of random peptides for specific sequences that interact with the NHE-RF PDZ domains and found that NHE-RF-PDZ1 is capable of binding to the CFTR C-terminus. The specific and tight association suggests a potential regulatory role of NHE-RF in cystic fibrosis transmembrane conductance regulator (CFTR) function.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
May
|
pubmed:issn |
0014-5793
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pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
1
|
pubmed:volume |
427
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
103-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9613608-Amino Acid Sequence,
pubmed-meshheading:9613608-Animals,
pubmed-meshheading:9613608-Consensus Sequence,
pubmed-meshheading:9613608-Cystic Fibrosis Transmembrane Conductance Regulator,
pubmed-meshheading:9613608-Drosophila Proteins,
pubmed-meshheading:9613608-Insect Proteins,
pubmed-meshheading:9613608-Mice,
pubmed-meshheading:9613608-Molecular Sequence Data,
pubmed-meshheading:9613608-Phosphoproteins,
pubmed-meshheading:9613608-Protein Conformation,
pubmed-meshheading:9613608-Sequence Alignment,
pubmed-meshheading:9613608-Sequence Homology, Amino Acid,
pubmed-meshheading:9613608-Sodium-Hydrogen Antiporter
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pubmed:year |
1998
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pubmed:articleTitle |
Peptide binding consensus of the NHE-RF-PDZ1 domain matches the C-terminal sequence of cystic fibrosis transmembrane conductance regulator (CFTR).
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pubmed:affiliation |
Department of Physiology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|