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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1998-6-15
|
| pubmed:abstractText |
We developed a competitive enzyme immunoassay for detection of rat P-selectin and examined temporal changes of plasma P-selectin levels in an endotoxin-induced injury model in rats. Soluble P-selectin was detected in rat plasma after intravenous administration of lipopolysaccharide (LPS), and increased to a maximum level of five-fold over baseline after 24 hours. Plasma P-selectin was partially purified by gelfiltration and was identified as a 122 kDa band by Western blotting. Using the ELISA system we developed, monitoring of plasma P-selectin has become possible in the rat.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
19
|
| pubmed:volume |
246
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
414-7
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9610374-Animals,
pubmed-meshheading:9610374-Binding, Competitive,
pubmed-meshheading:9610374-Disease Models, Animal,
pubmed-meshheading:9610374-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:9610374-Injections, Intravenous,
pubmed-meshheading:9610374-Kinetics,
pubmed-meshheading:9610374-Lipopolysaccharides,
pubmed-meshheading:9610374-Male,
pubmed-meshheading:9610374-P-Selectin,
pubmed-meshheading:9610374-Rats,
pubmed-meshheading:9610374-Rats, Sprague-Dawley,
pubmed-meshheading:9610374-Reference Standards,
pubmed-meshheading:9610374-Solubility
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Increased plasma P-selectin induced by intravenous administration of endotoxin in rats.
|
| pubmed:affiliation |
Sumitomo Pharmaceuticals Research Center, Osaka, Japan.
|
| pubmed:publicationType |
Journal Article
|