Linked Life Data

9609708

Source:http://linkedlifedata.com/resource/pubmed/id/9609708

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
1998-6-22
pubmed:abstractText
The tetracenomycin polyketide synthase (TCM PKS), a type II complex, produces TCM F2, a precursor of TCM C in Streptomyces glaucescens, and consists of at least the TcmK, -L, -M, and -N proteins. The TcmK/TcmL ketosynthase subunits were purified from overexpression of their genes in Streptomyces lividans. TcmK (calculated molecular mass 45 kd) and TcmL (calculated molecular mass 42 kd) function as a heterodimeric alphabeta complex based on observing that only the purified complex complemented TCM PKS activity in protein extracts made from strains bearing tcmK or tcmL deletion mutants to make TCM F2 in vitro, and that the molecular mass of the purified complex was 90 kd as estimated by gel filtration chromatography. The TCM PKS activity was reconstituted with purified protein components, indicating that the minimal set of proteins required to make TCM F2 included the ketosynthase complex (TcmKL), an acyl carrier protein (TcmM), a malonyl CoA:ACP acyltransferase (MAT), and a cyclase (TcmN). The MAT was required to catalyze the transacylation between malonyl-CoA and TcmM, although a relatively slow spontaneous transacylation also occurred in a reaction without the MAT. Acetyl-CoA, the proposed starter unit for the TCM PKS, was not required for the production of TCM F2 in vitro, although it could be incorporated into this polyketide to a small extent. TcmJ, a PKS protein without a known function, greatly increased the production of TCM F2 but could not replace TcmN as a cyclase in the reconstituted system.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Acetyl Coenzyme A, http://linkedlifedata.com/resource/pubmed/chemical/Acyl Carrier Protein, http://linkedlifedata.com/resource/pubmed/chemical/Acyl Coenzyme A, http://linkedlifedata.com/resource/pubmed/chemical/Aldehyde-Lyases, http://linkedlifedata.com/resource/pubmed/chemical/Anthracenes, http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes, http://linkedlifedata.com/resource/pubmed/chemical/TcmJ protein, Streptomyces..., http://linkedlifedata.com/resource/pubmed/chemical/TcmN protein, Streptomyces..., http://linkedlifedata.com/resource/pubmed/chemical/methylmalonyl-coenzyme A, http://linkedlifedata.com/resource/pubmed/chemical/tetracenomycin F2, http://linkedlifedata.com/resource/pubmed/chemical/tetracenomycin F2 cyclase...
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8132-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Reconstitution of the iterative type II polyketide synthase for tetracenomycin F2 biosynthesis.
pubmed:affiliation
School of Pharmacy, Department of Bacteriology, University of Wisconsin, Madison 53706, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.