Linked Life Data

9608424

Source:http://linkedlifedata.com/resource/pubmed/id/9608424

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-8-3
pubmed:abstractText
High performance liquid chromatography (HPLC) with an on-line flow-through radioactivity detector was used to monitor the metabolism of cytokinins ([3H]6-benzylaminopurine and [3H]6-benzylaminopurine riboside) after their incorporation into wheat seedlings. The production and conversion of individual metabolites was assayed within a short time interval (0.5-3 h). Extraction recoveries from plant tissue proved to be 85%. The uptake of both cytokinins was very rapid and differences in their metabolism were already perceptible after 30 min. The individual metabolites were identified as adenine (Ade), adenosine (Ado), benzyladenine-9-glucoside (BA-9G), 6-benzyladenine (BA) and benzyladenosine (BAR). The method is very fast, sensitive and very useful for metabolic studies. The detection limit was 40 pg (220 Bq) for BA at the level of 2 ng ml-1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
39-44
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Study of cytokinin metabolism using HPLC with radioisotope detection.
pubmed:affiliation
Isotope Laboratory, Institute of Experimental Botany, Prague, Czech Republic.
pubmed:publicationType
Journal Article