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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1998-8-5
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pubmed:abstractText |
A simple procedure for elution in water of bacterial lipopolysaccharides (LPS) from sodium dodecyl sulfate-polyacrylamide gels is described. It consists of the combination of three principal steps: first, highly sensitive on-gel LPS detection (1-10 ng/band) with zinc-imidazole (negative or reverse staining); second, washing of the individual LPS band in a solution of a zinc-complexing agent (e.g., 100 mM EDTA); and finally, elution of the LPS (100-200 microliters water for a 0.5-microgram LPS band) from gel microparticles for 3 h at room temperature. Using this procedure, we have successfully eluted a variety of LPS forms from Bordetella pertussis, Escherichia coli 0111:B4, E. coli K-235, Salmonella enteritidis, and Pseudomonas aeruginosa. Elution recovery of rough or semismooth LPS was about 70-80%, while that of smooth LPS was only about 10%. Eluted LPS was biologically active as tested by limulus amebocyte lysate and TNF-alpha assays.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0003-2697
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
259
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
162-5
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:9606157-Acrylic Resins,
pubmed-meshheading:9606157-Bordetella pertussis,
pubmed-meshheading:9606157-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9606157-Escherichia coli,
pubmed-meshheading:9606157-Humans,
pubmed-meshheading:9606157-Limulus Test,
pubmed-meshheading:9606157-Lipopolysaccharides,
pubmed-meshheading:9606157-Microchemistry,
pubmed-meshheading:9606157-Pseudomonas aeruginosa,
pubmed-meshheading:9606157-Salmonella enteritidis,
pubmed-meshheading:9606157-Tumor Necrosis Factor-alpha
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pubmed:year |
1998
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pubmed:articleTitle |
Elution of lipopolysaccharides from polyacrylamide gels.
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pubmed:affiliation |
Center for Genetic Engineering and Biotechnology, Havana, Cuba.
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pubmed:publicationType |
Journal Article
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