Linked Life Data

9606138

Source:http://linkedlifedata.com/resource/pubmed/id/9606138

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-8-5
pubmed:abstractText
O-linked oligosaccharide-alditols were analyzed by a combination of high-performance liquid chromatography (HPLC) and electrospray-tandem mass spectrometry (ESI-MS/MS). First, oligosaccharide-alditols were treated with sodium meta-periodate under conditions where core N-acetylgalactosaminitol is specifically degraded. The resulting fragments were labeled with 2-aminopyridine and purified on a reversed-phase column. Pyridylamino oligosaccharides yielded protonated molecular ions in positive-ion ES-MS and gave Y-series sequence ions, arising from glycosidic cleavages, by ESI-tandem mass spectrometry. Information on sugar sequence and branching of oligosaccharides linked at C6 and C3 to the N-acetylgalactosaminitol can be obtained. A systematic study of various oligosaccharide-alditols demonstrated that this approach constitutes a powerful tool for the structural characterization of O-glycans available only in limited quantities.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
259
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
16-27
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Structural analysis of O-linked oligosaccharide-alditols by electrospray-tandem mass spectrometry after mild periodate oxidation and derivatization with 2-aminopyridine.
pubmed:affiliation
Laboratoire de Chimie Biologique, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't