Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1998-6-10
pubmed:abstractText
The activation status of the ras pathway was studied in eight ovarian tumor cell lines. Three biochemical parameters indicative of ras activation were tested: (a) the ratio of the ras-GTP:ras-GDP complex; (b) the activity of mitogen-activated protein kinases p42/p44; and (c) ets-2 phosphorylation at position threonine 72, a mitogen-activated protein kinase phosphorylation site in vivo. Four of the ovarian tumor cell lines had an activated ras pathway by these three parameters, whereas only one of these contained a mutated ras gene. In addition, ras/ets-2 responsive genes such as the urokinase plasminogen activator (uPA) were activated in these four cell lines. Transient transfection assays indicated that the compound ets-AP1 oncogene responsive enhancer present in the uPA gene was the target of ras signaling in ovarian tumor cells and that the combination of activated ras and ets-2 could superactivate the uPA enhancer element. Coexpression of the dominant-negative ras-Asn17 cDNA gene abrogated activity of this uPA element in ovarian tumor cells. These data indicate that ets-2 is a nuclear target of ras action in ovarian tumor cell lines and that ras signaling pathways may be activated in ovarian cancer by mechanisms independent of direct genetic damage to ras genes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents, http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent..., http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/ERF protein, human, http://linkedlifedata.com/resource/pubmed/chemical/ETS2 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Genistein, http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Triphosphate, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Protein c-ets-2, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Urokinase-Type Plasminogen Activator
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2253-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9605774-Antineoplastic Agents, pubmed-meshheading:9605774-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:9605774-Carcinoma, pubmed-meshheading:9605774-DNA-Binding Proteins, pubmed-meshheading:9605774-Enzyme Activation, pubmed-meshheading:9605774-Female, pubmed-meshheading:9605774-Gene Expression Regulation, pubmed-meshheading:9605774-Genes, ras, pubmed-meshheading:9605774-Genistein, pubmed-meshheading:9605774-Guanosine Triphosphate, pubmed-meshheading:9605774-Humans, pubmed-meshheading:9605774-Ovarian Neoplasms, pubmed-meshheading:9605774-Phosphorylation, pubmed-meshheading:9605774-Proto-Oncogene Protein c-ets-2, pubmed-meshheading:9605774-Proto-Oncogene Proteins, pubmed-meshheading:9605774-Repressor Proteins, pubmed-meshheading:9605774-Trans-Activators, pubmed-meshheading:9605774-Transcription Factors, pubmed-meshheading:9605774-Tumor Cells, Cultured, pubmed-meshheading:9605774-Urokinase-Type Plasminogen Activator
pubmed:year
1998
pubmed:articleTitle
Activation of the ras-mitogen-activated protein kinase pathway and phosphorylation of ets-2 at position threonine 72 in human ovarian cancer cell lines.
pubmed:affiliation
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.