Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1998-6-11
pubmed:abstractText
IFN-stimulated gene factor 3 (ISGF3) mediates transcriptional activation of IFN-sensitive genes (ISGs). The component subunits of ISGF3, STAT1alphabeta, STAT2, and p48-ISGF3gamma, are tyrosine phosphorylated before their assembly into a complex. Subsequently, the ISGF3 complex is translocated to the nucleus. We have recently established that the responsiveness of human melanoma cell lines to type I IFNs correlates directly with their intracellular levels of ISGF3 components, particularly STAT1. In the present study, we show that pretreating IFN-resistant melanoma cell lines with IFN-gamma (IFN-gamma priming) before stimulation with type I IFN also results in increased levels of ISGF3 components and enhanced DNA-binding activation of ISGF3. In addition, IFN-gamma priming of IFN-resistant melanoma cell lines increased expression of type I IFN-induced ISG products, including ISG54, 2'-5'-oligoadenylate synthase, HLA class I, B7-1, and ICAM-1 Ags. Furthermore, IFN-gamma priming enhanced the antiviral effect of IFN-beta on the IFN-resistant melanoma cell line, MM96. These results support a role for IFN-gamma priming in up-regulating ISGF3, thereby augmenting the responsiveness of IFN-resistant melanoma cell lines to type I IFN and providing a molecular basis and justification for using sequential IFN therapy, as proposed by others, to enhance the use of IFNs in the treatment of melanoma.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/IRF9 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Interferon Type I, http://linkedlifedata.com/resource/pubmed/chemical/Interferon-Stimulated Gene Factor 3, http://linkedlifedata.com/resource/pubmed/chemical/Interferon-Stimulated Gene Factor..., http://linkedlifedata.com/resource/pubmed/chemical/Interferon-alpha, http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/STAT1 Transcription Factor, http://linkedlifedata.com/resource/pubmed/chemical/STAT1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/STAT2 Transcription Factor, http://linkedlifedata.com/resource/pubmed/chemical/STAT2 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/interferon alfa-2a
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
160
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5475-84
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:9605150-Adjuvants, Immunologic, pubmed-meshheading:9605150-DNA-Binding Proteins, pubmed-meshheading:9605150-Drug Resistance, Neoplasm, pubmed-meshheading:9605150-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:9605150-Gene Expression Regulation, Neoplastic, pubmed-meshheading:9605150-Humans, pubmed-meshheading:9605150-Interferon Type I, pubmed-meshheading:9605150-Interferon-Stimulated Gene Factor 3, pubmed-meshheading:9605150-Interferon-Stimulated Gene Factor 3, gamma Subunit, pubmed-meshheading:9605150-Interferon-alpha, pubmed-meshheading:9605150-Interferon-gamma, pubmed-meshheading:9605150-Melanoma, pubmed-meshheading:9605150-Recombinant Proteins, pubmed-meshheading:9605150-STAT1 Transcription Factor, pubmed-meshheading:9605150-STAT2 Transcription Factor, pubmed-meshheading:9605150-Signal Transduction, pubmed-meshheading:9605150-Trans-Activators, pubmed-meshheading:9605150-Transcription Factors, pubmed-meshheading:9605150-Tumor Cells, Cultured, pubmed-meshheading:9605150-Up-Regulation
pubmed:year
1998
pubmed:articleTitle
IFN-gamma priming up-regulates IFN-stimulated gene factor 3 (ISGF3) components, augmenting responsiveness of IFN-resistant melanoma cells to type I IFNs.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
pubmed:publicationType
Journal Article