Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5-6
pubmed:dateCreated
1998-8-6
pubmed:abstractText
beta neuregulins (also called NDF, GGF, ARIA, and heregulins) are neuron-derived molecules that are likely to be responsible for Schwann cell precursor survival, proliferation, and maturation in vivo and in vitro. Although the receptors to which beta neuregulins bind have been defined, little is known about the transcription factors these important ligands activate. Using antibodies, quantitative imaging methods and Western blotting, we show that beta neuregulin induces a high level of phosphorylation of the transcription factor cyclic AMP response element binding protein (CREB) on Ser-133 in cultured rat Schwann cells and that the phosphorylation is prolonged over several hours. In contrast, neurotrophins, CNTF, FGF-2, EGF, and TGF beta induce little or no phosphorylation of CREB despite the fact that receptors for these factors are present on Schwann cells. As expected CREB phosphorylation was detected following cAMP elevation, and it was also induced by elevation of cytoplasmic Ca2+, endothelin 1, and PDGF-BB. The signal was lower than that seen in response to beta neuregulin, and transient, unlike the sustained CREB activation induced by beta neuregulin. Our results suggest that the sustained phosphorylation of CREB on Ser-133 may contribute to the broad spectrum of effects that beta neuregulins have on cells of the Schwann cell lineage and that the CREB pathway may be important for transduction of neuregulin signals in Schwann cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1044-7431
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
309-22
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9604209-Animals, pubmed-meshheading:9604209-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:9604209-Cells, Cultured, pubmed-meshheading:9604209-Cyclic AMP Response Element-Binding Protein, pubmed-meshheading:9604209-Dose-Response Relationship, Drug, pubmed-meshheading:9604209-Drug Synergism, pubmed-meshheading:9604209-Endothelins, pubmed-meshheading:9604209-Forskolin, pubmed-meshheading:9604209-Glycoproteins, pubmed-meshheading:9604209-Insulin-Like Growth Factor I, pubmed-meshheading:9604209-Nerve Growth Factors, pubmed-meshheading:9604209-Neuregulins, pubmed-meshheading:9604209-Neuroglia, pubmed-meshheading:9604209-Neurons, pubmed-meshheading:9604209-Phosphorylation, pubmed-meshheading:9604209-Platelet-Derived Growth Factor, pubmed-meshheading:9604209-Rats, pubmed-meshheading:9604209-Rats, Sprague-Dawley, pubmed-meshheading:9604209-Schwann Cells, pubmed-meshheading:9604209-Serine, pubmed-meshheading:9604209-Time Factors
pubmed:year
1998
pubmed:articleTitle
The neuron-glia signal beta neuregulin induces sustained CREB phosphorylation on Ser-133 in cultured rat Schwann cells.
pubmed:affiliation
Department of Anatomy and Developmental Biology, University College London, England.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't