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rdf:type |
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lifeskim:mentions |
umls-concept:C0006674,
umls-concept:C0013879,
umls-concept:C0017337,
umls-concept:C0020792,
umls-concept:C0022646,
umls-concept:C0042890,
umls-concept:C0086418,
umls-concept:C0332307,
umls-concept:C0597484,
umls-concept:C0851285,
umls-concept:C0851827,
umls-concept:C0890466,
umls-concept:C1420185,
umls-concept:C1701901
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pubmed:issue |
23
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pubmed:dateCreated |
1998-7-16
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pubmed:abstractText |
Vitamin D is an important regulator of phosphate homeostasis. The effects of vitamin D on the expression of renal Na+-dependent inorganic phosphate (Pi) transporters (types I and II) were investigated. In vitamin D-deficient rats, the amounts of type II Na+-dependent Pi transporter (NaPi-2) protein and mRNA were decreased in the juxtamedullary kidney cortex, but not in the superficial cortex, compared with control rats. The administration of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) to vitamin D-deficient rats increased the initial rate of Pi uptake as well as the amounts of NaPi-2 mRNA and protein in the juxtamedullary cortex. The transcriptional activity of a luciferase reporter plasmid containing the promoter region of the human type II Na+-dependent Pi transporter NaPi-3 gene was increased markedly by 1,25-(OH)2D3 in COS-7 cells expressing the human vitamin D receptor. A deletion and mutation analysis of the NaPi-3 gene promoter identified the vitamin D-responsive element as the sequence 5'-GGGGCAGCAAGGGCA-3' nucleotides -1977 to -1963 relative to the transcription start site. This element bound a heterodimer of the vitamin D receptor and retinoid X receptor, and it enhanced the basal transcriptional activity of the promoter of the herpes simplex virus thymidine kinase gene in an orientation-independent manner. Thus, one mechanism by which vitamin D regulates Pi homeostasis is through the modulation of the expression of type II Na+-dependent Pi transporter genes in the juxtamedullary kidney cortex.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcitriol,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphate-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Calcitriol,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Retinoic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoid X Receptors,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:ChikamoriMM,
pubmed-author:IemoriYY,
pubmed-author:KidoSS,
pubmed-author:KobayashiTT,
pubmed-author:MiyamotoKK,
pubmed-author:MoritaKK,
pubmed-author:OkanoTT,
pubmed-author:SegawaHH,
pubmed-author:TakedaEE,
pubmed-author:TaketaniYY,
pubmed-author:TanakaKK,
pubmed-author:TatsumiSS,
pubmed-author:TsugawaNN,
pubmed-author:YamamotoHH
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14575-81
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9603973-Animals,
pubmed-meshheading:9603973-COS Cells,
pubmed-meshheading:9603973-Calcitriol,
pubmed-meshheading:9603973-Carrier Proteins,
pubmed-meshheading:9603973-DNA-Binding Proteins,
pubmed-meshheading:9603973-Gene Expression Regulation,
pubmed-meshheading:9603973-Humans,
pubmed-meshheading:9603973-Immunohistochemistry,
pubmed-meshheading:9603973-Kidney Cortex,
pubmed-meshheading:9603973-Male,
pubmed-meshheading:9603973-Microvilli,
pubmed-meshheading:9603973-Nuclear Proteins,
pubmed-meshheading:9603973-Phosphate-Binding Proteins,
pubmed-meshheading:9603973-Phosphates,
pubmed-meshheading:9603973-Promoter Regions, Genetic,
pubmed-meshheading:9603973-RNA, Messenger,
pubmed-meshheading:9603973-Rats,
pubmed-meshheading:9603973-Rats, Wistar,
pubmed-meshheading:9603973-Receptors, Calcitriol,
pubmed-meshheading:9603973-Receptors, Retinoic Acid,
pubmed-meshheading:9603973-Retinoid X Receptors,
pubmed-meshheading:9603973-Transcription Factors,
pubmed-meshheading:9603973-Vitamin D Deficiency
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pubmed:year |
1998
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pubmed:articleTitle |
Regulation of type II renal Na+-dependent inorganic phosphate transporters by 1,25-dihydroxyvitamin D3. Identification of a vitamin D-responsive element in the human NAPi-3 gene.
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pubmed:affiliation |
Department of Clinical Nutrition, School of Medicine, University of Tokushima, Tokushima 770-8503, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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