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pubmed-article:9603896pubmed:abstractTextMotile swarmer cells of Hyphomicrobium strain W1-1B displayed positive chemotactic responses toward methylamine, dimethylamine, and trimethylamine but did not display significant chemotactic responses towards methanol and arginine. Electron micrographs of negatively stained intact flagellar filaments indicated a novel striated surface pattern. The flagella were composed of two proteins of 39 and 41 kDa. Neither protein was a glycoprotein as determined by Schiff's staining and by enzyme immunoassay. Protein fingerprints visualized from silver-stained polyacrylamide gels and Western blots of protease-digested samples indicated that the two proteins were similar but not identical. Monoclonal antibodies prepared to the complex flagella of Rhizobium meliloti cross-reacted with the striated flagella of Hyphomicrobium strain W1-1B; however, these antibodies did not cross-react with smooth-surface flagella. These results suggest that complex and striated flagella possess homologous epitope regions.lld:pubmed
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pubmed-article:9603896pubmed:articleTitleCharacterization of chemotactic responses and flagella of Hyphomicrobium strain W1-1B.lld:pubmed
pubmed-article:9603896pubmed:affiliationDepartment of Microbiology, The Ohio State University, Columbus, Ohio 43210-1292, USA.lld:pubmed
pubmed-article:9603896pubmed:publicationTypeJournal Articlelld:pubmed
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