rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
11
|
pubmed:dateCreated |
1998-6-25
|
pubmed:abstractText |
Motile swarmer cells of Hyphomicrobium strain W1-1B displayed positive chemotactic responses toward methylamine, dimethylamine, and trimethylamine but did not display significant chemotactic responses towards methanol and arginine. Electron micrographs of negatively stained intact flagellar filaments indicated a novel striated surface pattern. The flagella were composed of two proteins of 39 and 41 kDa. Neither protein was a glycoprotein as determined by Schiff's staining and by enzyme immunoassay. Protein fingerprints visualized from silver-stained polyacrylamide gels and Western blots of protease-digested samples indicated that the two proteins were similar but not identical. Monoclonal antibodies prepared to the complex flagella of Rhizobium meliloti cross-reacted with the striated flagella of Hyphomicrobium strain W1-1B; however, these antibodies did not cross-react with smooth-surface flagella. These results suggest that complex and striated flagella possess homologous epitope regions.
|
pubmed:commentsCorrections |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Arginine,
http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Flagellin,
http://linkedlifedata.com/resource/pubmed/chemical/Methanol,
http://linkedlifedata.com/resource/pubmed/chemical/Methylamines,
http://linkedlifedata.com/resource/pubmed/chemical/Serine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/glutamyl endopeptidase
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0021-9193
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
180
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
3003-6
|
pubmed:dateRevised |
2009-11-18
|
pubmed:meshHeading |
pubmed-meshheading:9603896-Antibodies, Bacterial,
pubmed-meshheading:9603896-Antibodies, Monoclonal,
pubmed-meshheading:9603896-Antigens, Bacterial,
pubmed-meshheading:9603896-Arginine,
pubmed-meshheading:9603896-Bacteria,
pubmed-meshheading:9603896-Chemotactic Factors,
pubmed-meshheading:9603896-Chemotaxis,
pubmed-meshheading:9603896-Cross Reactions,
pubmed-meshheading:9603896-Flagella,
pubmed-meshheading:9603896-Flagellin,
pubmed-meshheading:9603896-Methanol,
pubmed-meshheading:9603896-Methylamines,
pubmed-meshheading:9603896-Serine Endopeptidases
|
pubmed:year |
1998
|
pubmed:articleTitle |
Characterization of chemotactic responses and flagella of Hyphomicrobium strain W1-1B.
|
pubmed:affiliation |
Department of Microbiology, The Ohio State University, Columbus, Ohio 43210-1292, USA.
|
pubmed:publicationType |
Journal Article
|