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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6681
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pubmed:dateCreated |
1998-6-4
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pubmed:abstractText |
Initiation of transcription of a gene from a core promoter region by RNA polymerase II requires the assembly of several initiation factors to form a preinitiation complex. Assembly of this complex is thought to be nucleated exclusively by the sequence-specific binding of the TFIID transcription factor complex, which is composed of the TATA-binding protein (TBP) and TBP-associated factors (TAF(II)s), to the different promoters. Here we isolate and characterize a new multiprotein complex that does not contain either TBP or a TBP-like factor but is composed of several TAF(II)s and other proteins. This complex can replace TFIID on both TATA-containing and TATA-lacking promoters in in vitro transcription assays. Moreover, an anti-TBP antibody that inhibits TBP- and TFIID-dependent transcription does not inhibit activity of this new complex. These results indicate that TBP-free RNA polymerase II mediated transcription may be able to occur in mammalian cells and that multiple preinitiation complexes may play an important role in regulating gene expression.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
393
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
187-91
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9603525-DNA Footprinting,
pubmed-meshheading:9603525-DNA-Binding Proteins,
pubmed-meshheading:9603525-HeLa Cells,
pubmed-meshheading:9603525-Humans,
pubmed-meshheading:9603525-RNA Polymerase II,
pubmed-meshheading:9603525-TATA-Binding Protein Associated Factors,
pubmed-meshheading:9603525-TATA-Box Binding Protein,
pubmed-meshheading:9603525-Transcription, Genetic,
pubmed-meshheading:9603525-Transcription Factor TFIID,
pubmed-meshheading:9603525-Transcription Factors,
pubmed-meshheading:9603525-Transcription Factors, TFII,
pubmed-meshheading:9603525-Transcriptional Activation
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pubmed:year |
1998
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pubmed:articleTitle |
Function of TAF(II)-containing complex without TBP in transcription by RNA polymerase II.
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pubmed:affiliation |
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Illkirch, CU de Strasbourg, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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