Linked Life Data

9603250

Source:http://linkedlifedata.com/resource/pubmed/id/9603250

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1998-7-21
pubmed:abstractText
Apoptosis of granulosa cells is the cellular mechanism of ovarian follicular atresia, and cytokines have been implicated as potential atretogenic factors. We therefore investigated the possible role of the cytokine Fas ligand (FasL) and its receptor Fas in apoptosis during ovarian follicular atresia induced by gonadotropin withdrawal. Immature rats pretreated with eCG were injected 24 h later with an antiserum generated against eCG (antibody group) or preimmune rabbit serum (control), and ovaries were removed 1 and 24 h after treatment. The eCG antiserum caused a dose-dependent inhibition of the significant increase in ovarian weight observed between 24 and 48 h after eCG treatment. In situ detection of fragmented DNA in histological sections identified cell death in atretic but not healthy small and medium-sized antral follicles of the antibody group. Cell death was distributed in a scattered pattern throughout the granulosa cell layer of small atretic follicles but was localized primarily in granulosa cells lining the antral cavity of atretic medium antral follicles. Immunohistochemistry of adjacent histological sections revealed intense positive immunostaining for Fas and FasL in granulosa cells of atretic small and medium antral follicles in a pattern coincidental to the localization of cell death. Intense FasL staining was evident in the theca cells of healthy small antral follicles. An increase in low molecular weight DNA (DNA "ladders") indicative of apoptosis was evident in granulosa cells of the antibody group. Western analysis demonstrated increased levels of both Fas and FasL in the granulosa cells of the antibody group. These results demonstrate that both Fas and FasL are present in ovarian granulosa cells and that FasL may be the signal that induces granulosa cell apoptosis during atresia at the penultimate stage of ovarian follicular development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1170-6
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:9603250-Animals, pubmed-meshheading:9603250-Antigens, CD95, pubmed-meshheading:9603250-Apoptosis, pubmed-meshheading:9603250-Blotting, Western, pubmed-meshheading:9603250-Chorionic Gonadotropin, pubmed-meshheading:9603250-DNA, pubmed-meshheading:9603250-DNA Fragmentation, pubmed-meshheading:9603250-Fas Ligand Protein, pubmed-meshheading:9603250-Female, pubmed-meshheading:9603250-Granulosa Cells, pubmed-meshheading:9603250-Histocytochemistry, pubmed-meshheading:9603250-Ligands, pubmed-meshheading:9603250-Luminescent Measurements, pubmed-meshheading:9603250-Membrane Glycoproteins, pubmed-meshheading:9603250-Organ Size, pubmed-meshheading:9603250-Ovarian Follicle, pubmed-meshheading:9603250-Ovary, pubmed-meshheading:9603250-Rabbits, pubmed-meshheading:9603250-Rats, pubmed-meshheading:9603250-Rats, Sprague-Dawley
pubmed:year
1998
pubmed:articleTitle
Granulosa cell apoptosis induced at the penultimate stage of follicular development is associated with increased levels of Fas and Fas ligand in the rat ovary.
pubmed:affiliation
Department of Obstetrics & Gynecology, University of Ottawa, Ottawa Civic Hospital Loeb Research Institute, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't