Linked Life Data

9602438

Source:http://linkedlifedata.com/resource/pubmed/id/9602438

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-7-31
pubmed:abstractText
Arterial injury results in the elaboration of pro-inflammatory substances including cytokines and peptide growth factors which act to modify vascular smooth muscle (VSMC) proliferation and migration with resultant vessel stenosis. Produced by T-lymphocytes and macrophages, interleukin-10 (IL-10) is an anti-inflammatory cytokine in several cell lines. We hypothesized that IL-10 may participate in vascular remodeling by inhibiting VSMC proliferation. Human aortic VSMCs were isolated and cultured. Proliferation assays were performed to determine the effect of the effect of IL-10 on (1) unstimulated, (2) cytokine (tumor necrosis factor-alpha: TNF alpha)-stimulated, and (3) growth factor (basic fibroblast growth factor: bFGF)-stimulated VSMC proliferation. Compared to control, both TNF alpha and bFGF-stimulated VSMC proliferation (P < 0.002). IL-10 alone had no effect on cell growth. However, with TNF alpha or bFGF-stimulation, physiologic doses of IL-10 inhibited both VSMC DNA synthesis and VSMC growth (P < 0.001). Furthermore, IL-10 was effective in inhibiting TNF alpha-induced proliferation at a dose as low as 10 fg/ml (P < 0.001) and bFGF-induced proliferation at a dose as low as 1 pg/ml (P < 0.001). In conclusion, TNF alpha and bFGF stimulate human VSMC growth. IL-10 potently abrogates the proliferative response to these atherogenic mitogens. IL-10 might represent an endogenous source of immune-mediated atherprotection and when given exogenously, may prove to be a novel therapeutic agent in regulating vessel wall remodeling following vascular injury.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-2828
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
889-96
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Interleukin-10 inhibits human vascular smooth muscle proliferation.
pubmed:affiliation
Department of Surgery, University of Colorado Health Sciences Center, Denver 80262, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't