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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1998-6-8
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pubmed:abstractText |
The HIV-1 protein Rev regulates the cytoplasmic levels of incompletely spliced HIV-1 mRNAs. The plasmid pSVc21, which contains a HIV-1 provirus, was introduced into COS cells by transient transfection. Simultaneous detection of HIV-1 RNAs and Rev proteins produced in transfected cells was then performed in order to determine the relative distribution of these two components. HIV-1 RNAs and the Rev protein localized to the same areas of the nucleoplasm, implying that these locations represent sites where Rev interacts with its target RNAs. Using a monoclonal antibody targeted to the splicing factor SC-35 it was demonstrated that the sites where HIV-1 mRNAs and Rev were detected often contained weak anti-SC-35 staining, whereas little RNA and Rev were found in strongly labeled SC-35-containing speckles. The same distribution of HIV-1 RNAs relative to SC-35 was also seen in transfected HeLa cells and in primary human lymphocytes infected with HIV-1 primary isolates. In addition, transiently expressed intron-containing beta-globin RNAs were shown to distribute to weak anti-SC-35 staining in a manner similar to that of HIV-1 RNAs. The findings suggest that Rev and HIV-1 RNAs interact at putative sites of mRNA transcription and splicing.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, rev,
http://linkedlifedata.com/resource/pubmed/chemical/Globins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/SRSF2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/rev Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
244
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
473-82
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pubmed:dateRevised |
2011-9-27
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pubmed:meshHeading |
pubmed-meshheading:9601515-Animals,
pubmed-meshheading:9601515-Antibodies, Monoclonal,
pubmed-meshheading:9601515-Binding Sites,
pubmed-meshheading:9601515-COS Cells,
pubmed-meshheading:9601515-Cell Nucleus,
pubmed-meshheading:9601515-Gene Products, rev,
pubmed-meshheading:9601515-Globins,
pubmed-meshheading:9601515-HIV-1,
pubmed-meshheading:9601515-Humans,
pubmed-meshheading:9601515-In Situ Hybridization, Fluorescence,
pubmed-meshheading:9601515-Introns,
pubmed-meshheading:9601515-Nuclear Proteins,
pubmed-meshheading:9601515-RNA, Messenger,
pubmed-meshheading:9601515-RNA, Viral,
pubmed-meshheading:9601515-RNA Splicing,
pubmed-meshheading:9601515-Ribonucleoproteins,
pubmed-meshheading:9601515-Subcellular Fractions,
pubmed-meshheading:9601515-Transfection,
pubmed-meshheading:9601515-rev Gene Products, Human Immunodeficiency Virus
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pubmed:year |
1998
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pubmed:articleTitle |
Subcellular localization of human immunodeficiency virus type 1 RNAs, Rev, and the splicing factor SC-35.
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pubmed:affiliation |
Department of Microbiology and Immunology, Gade Institute, Bergen High Technology Center, University of Bergen, Norway. Stig.Boe@rmf.ulb.no
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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