|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1998-6-16
|
|
pubmed:abstractText |
Bradykinin (BK) B2 receptor signaling involves activation of phospholipase C (PLC). PLC activation by other receptors consists of either allosteric activation of PLC beta isoforms by G-proteins or tyrosine phosphorylation of PLC gamma isoforms. Because the B2 receptor is a G-protein-coupled receptor, it has been assumed that the receptor signals through PLC beta. In the present study, however, we have found that BK stimulation of IP3 production and the Ca2+ signal in endothelial cells is dependent on tyrosine phosphorylation. Furthermore, stimulation of B2 receptors in these cells is accompanied by a transient tyrosine phosphorylation of PLC gamma 1. Phosphorylation is correlated with increased IP3 production and association of PLC gamma 1 with the C-terminal intracellular domain of the B2 receptor. The B2 receptor can thus physically associate with intracellular proteins other than G-proteins. Activation of PLC gamma isoforms, rather than PLC beta isoforms, may, therefore, be primarily responsible for BK-stimulated IP3 generation in endothelial cells.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-amino-5-(4-methylphenyl)-7-(tert-b...,
http://linkedlifedata.com/resource/pubmed/chemical/Bradykinin,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase C gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrimidines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Angiotensin, Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Angiotensin, Type 2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Bradykinin B2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Angiotensin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Bradykinin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/src-Family Kinases
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0006-291X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
8
|
|
pubmed:volume |
246
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
70-5
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:9600070-Animals,
pubmed-meshheading:9600070-Bradykinin,
pubmed-meshheading:9600070-Cattle,
pubmed-meshheading:9600070-Cells, Cultured,
pubmed-meshheading:9600070-Endothelium, Vascular,
pubmed-meshheading:9600070-Enzyme Activation,
pubmed-meshheading:9600070-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:9600070-Isoenzymes,
pubmed-meshheading:9600070-Phospholipase C gamma,
pubmed-meshheading:9600070-Phosphorylation,
pubmed-meshheading:9600070-Pyrazoles,
pubmed-meshheading:9600070-Pyrimidines,
pubmed-meshheading:9600070-Receptor, Angiotensin, Type 1,
pubmed-meshheading:9600070-Receptor, Angiotensin, Type 2,
pubmed-meshheading:9600070-Receptor, Bradykinin B2,
pubmed-meshheading:9600070-Receptors, Angiotensin,
pubmed-meshheading:9600070-Receptors, Bradykinin,
pubmed-meshheading:9600070-Recombinant Fusion Proteins,
pubmed-meshheading:9600070-Signal Transduction,
pubmed-meshheading:9600070-Type C Phospholipases,
pubmed-meshheading:9600070-Tyrosine,
pubmed-meshheading:9600070-src-Family Kinases
|
|
pubmed:year |
1998
|
|
pubmed:articleTitle |
Bradykinin stimulates the tyrosine phosphorylation and bradykinin B2 receptor association of phospholipase C gamma 1 in vascular endothelial cells.
|
|
pubmed:affiliation |
Department of Pediatrics, Medical College of Georgia, Augusta 30912, USA. rvenema@mail.mcg.edu
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
